Lipopolysaccharide-Induced Activation of β2-Integrin Function in Macrophages Requires Irak Kinase Activity, p38 Mitogen- Activated Protein Kinase, and the Rap1 GTPase

Abstract

Lipopolysaccharide (LPS), a component of the outer membrane of gram-negative bacteria, is a potent activator of macrophages. Besides inducing many transcriptional pathways, LPS also elicits rapid morphological changes such as cell spreading. Here we have investigated the signaling pathway that controls macrophage β2-integrin-dependent spreading in response to LPS. We show that inhibition of the adapter protein MyD88, the interleukin-1 receptor-associated kinase Irak, the p38 mitogen-activated protein kinase, or the Ras-like GTPase Rap1 blocks LPS-induced spreading. In addition, Irak activates p38 and stimulates p38-dependent spreading. The activation of p38 by Irak requires Irak's kinase activity. We find that p38 controls spreading independently of its role in transcription but rather through activation of Rap1. Together, our results suggest that β2-integrin-dependent spreading of macrophages in response to LPS is controlled by a linear signaling pathway via MyD88, Irak, p38, and Rap1.

ACKNOWLEDGMENTS

We thank J. Bos, R. Davis, C. von Eichel-Streiber, D. Goeddel, S. Moss, M. Muzio, E. Nishida, H. Nishitoh, and A. Self for reagents and DNA constructs, and members of the lab for valuable discussion.

A.S. is the recipient of an EMBO long-term fellowship. E.C. is supported by the Wellcome Trust. A.H. thanks the Cancer Research Campaign (UK) for generous support.