Effect of the Addition of a Labile Gelatin Component on the Degradation and Solute Release Kinetics of a Stable PEG Hydrogel

Abstract

Characterization of the degradation mechanisms and resulting products of biodegradable materials is critical in understanding the behavior of the material including solute transport and biological response. Previous mathematical analyses of a semi-interpenetrating network (sIPN) containing both labile gelatin and a stable cross-linked poly(ethylene glycol) (PEG) network found that diffusion-based models alone were unable to explain the release kinetics of solutes from the system. In this study, degradation of the sIPN and its effect on solute release and swelling kinetics were investigated. The kinetics of the primary mode of degradation, gelatin dissolution, was dependent on temperature, preparation methods, PEGdA and gelatin concentration, and the weight ratio between the gelatin and PEG. The gelatin dissolution rate positively correlated with both matrix swelling and the release kinetics of high-molecular-weight model compound, FITC-dextran. Coupled with previous in vitro studies, the kinetics of sIPN degradation provided insights into the time-dependent changes in cellular response including adhesion and protein expression. These results provide a facile guide in material formulation to control the delivery of high-molecular-weight compounds with concomitant modulation of cellular behavior.

Keywords:

• Degradation
• delivery vehicle
• gelatin
• hydrogel
• poly(ethylene glycol)
• semi-interpenetrating polymer network (sIPN)

Acknowledgements

The authors would like to thank Yao Fu and Nick Ladwig for their assistance with the gelatin dissolution study. This work was supported in part by NIH Grant R01 EB6613 and NIH 5R21NS063200-02.

Notes

aSignificantly different from the same formulation of sIPN polymerized at 4°C, P < 0.05.