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Journal of Adhesion Science and Technology Volume 26, 2012 - Issue 12-17: Electrowetting
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Articles

An ELISA Chip Based on an EWOD Microfluidic Platform

Liang Zhu State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing, 100084, P. R. China
,
Yanying Feng State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing, 100084, P. R. China
,
Xiongying Ye State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing, 100084, P. R. ChinaCorrespondence[email protected]
,
Jinyang Feng State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing, 100084, P. R. China
,
Yanbin Wu State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing, 100084, P. R. China
&
Zhaoying Zhou State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanology, Tsinghua University, Beijing, 100084, P. R. China
Pages 2113-2124 | Published online: 17 May 2012
 
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Abstract

We have developed an enzyme-linked immunosorbent assay (ELISA) chip based on a digital microfluidic platform, in which droplet generation and manipulation are implemented using the electrowetting on dielectrics (EWOD) effect. First, droplet transportability was experimentally tested on silicon test chips with different control electrodes for optimizing the EWOD-based droplet manipulation, and then tested on transparent indium-tin-oxide (ITO) chips. The latter were then used for the development of an ELISA chip, on which we carried out immunoassay with rat immunoglobulin G (IgG) and goat anti-rat IgG marked with horseradish peroxidase as an example. In driving the reagent solutions, the average velocity of droplets is up to 5–7 mm/s on the ELISA chip, and a chip-scaled immunoassay can be finished within 20 min by using colorimetric detection, with the volume of the sample and reagents of only 0.5–1 μl.

Acknowledgements

The research was supported by the National Natural Science Foundation of China (50730009, 50405001) and the Major State Basic Research Development Program (2007CB310504).

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