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Original Article

Bypassing the maturation arrest in myeloid cell line U937 by over-expression of microRNA-424

, , , , , & show all
Pages 298-302 | Published online: 12 Nov 2013

Figures & data

Figure 1. U937 cells at day 14 after transduction: untreated control culture (A); U937 cells infected with backbone of the vector (B); the group that transduced with RV-424 (C and D). The aggregated (C) and adherent (D) monocyte/macrophage-like phenotypes.

Figure 1. U937 cells at day 14 after transduction: untreated control culture (A); U937 cells infected with backbone of the vector (B); the group that transduced with RV-424 (C and D). The aggregated (C) and adherent (D) monocyte/macrophage-like phenotypes.

Figure 2. Expression analysis of monocyte markers using real-time PCR at days 7 and 14 after transduction.

Figure 2. Expression analysis of monocyte markers using real-time PCR at days 7 and 14 after transduction.

Figure 3. Expression analysis of surface markers CD11b and CD14 using flow cytometry: (A) distribution of cells by size and granularity; (B) negative control; (C) the group transduced by backbone of the vector; (D) the group transduced by RV-424.

Figure 3. Expression analysis of surface markers CD11b and CD14 using flow cytometry: (A) distribution of cells by size and granularity; (B) negative control; (C) the group transduced by backbone of the vector; (D) the group transduced by RV-424.

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