The prevalence of the platelet glycoprotein VI polymorphisms in patients with sticky platelet syndrome and ischemic stroke

Figures & data

Table 1. Characteristics of SPS patients and the control group

	SPS patients	Control group
Subjects (%)	71 (100)	77 (100)
Men (%)	24 (33)	34 (44)
Age [mean ± SD; years]*	49.2 ± 14.0**	35.9 ± 12.0
Type of SPS – patients (%)***
SPS type I	17 (24)	35.9 ± 12.0
SPS type II	52 (73)	–
SPS type III	2 (3)	–

SPS, sticky platelet syndrome.

*Age at the time of the first thromboembolic event.

**Statistically significant difference compared to the control group (P < 0.005).

***Classification according to the criteria of Mammen³ and Bick.²

Figure 1. Relationships between genotyped markers block in CEU HapMap population, SPS patients and the control group (LD plots).

Table 2. Characterization of the examined GP6 SNPs

SNP	Position on Ch. 19	Major/ minor allele	SNP sequence	5′-Forward primer-3′	5′-Reward primer-3′	RE
rs1654410	55524813	T/C	TCTGTC/TTTCTG	AGTACCTGAGAGTAGGCATGTGG	CTCCAAATGTAAGAATGGGTCAA	Mbo II
rs1671153	55527189	T/G	CATGGT/GGAGGC	CTTTGGACTGGCGGTGAT	CGGCCCATGCTTTTCTTA	Hph I
rs1654419	55535881	G/A	CGTCCG/ACCTTT	GGCTCTTTCCAGCATGTTTC	TTGTGTGAGGCGTGTTATCC	Cse I
rs11669150	55535972	T/C	TGAGAT/CGTTTG	TGCAGGCCGTAGTCTGGT	TGCTGCTTTGAGAACCACTG	Alw26 I
rs1613662	55536595	A/G	TACCGA/GGGAAG	AGGGGTCCGTGTACCTCA	AGGACCCACAGCGACAGA	Msp I
rs12610286	55541399	A/G	TTAACA/GTATTT	ATTAGGGTGGCGTGGCGTGAG	TGCATCCACAAAAGCCTAGA	Tai I
rs12610286	55541399	A/G	TTAACA/GTATTT	GGATGTAAAATTTAATTATTCCCAAA	AACGACCTACTCTTCAAATTTTC	HRM*
rs1654431	55549758	A/G	TAGCCA/GGCTCC	AGAATGCTCTCCTCCCTTCC	CAGGGAGTTTATGGGAGCAC	Alu I

RE, restriction endonuclease; SNP, single-nucleotide polymorphism.

*High-resolution melt (HRM) primers used for high-resolution melt analysis of rs12610286 heterozygotes in the check on process.

Table 3. Frequency of alleles of the GP6 SNPs in SPS patients and the control group

SNP	Major allele			Minor allele			Fisher's HWE P (SPS)	Fisher's HWE P (controls)	Fisher's exact P (allelic)	Fisher's exact P (Bonferroni)	OR (minor allele)	CI (95%)	Fisher's exact P (genotypic)	Fisher's exact P (Bonferroni)
	Allele	Freq	Allele	Allele	Freq (SPS)	Freq (controls)
rs1654410	T	0.535	0.506	C	0.465	0.494	0.812	0.499	0.643	1	0.891	0.564–1.407	0.660	1
rs1671153	T	0.901	0.903	G	0.099	0.097	1.000	1.000	1.000	1	1.014	0.470–2.182	1.000	1
rs1654419	G	0.887	0.903	A	0.113	0.097	0.586	1.000	0.707	1	1.177	0.558–2.477	0.690	1
rs11669150	T	0.746	0.721	C	0.254	0.279	0.529	0.778	0.693	1	0.877	0.522–1.469	0.859	1
rs1613662	A	0.894	0.922	G	0.106	0.078	1.000	1.000	0.427	1	1.398	0.630–3.097	0.403	1
rs12610286	A	0.627	0.682	G	0.373	0.318	0.021	0.437	0.330	1	1.276	0.789–2.062	0.304	1
rs1654431	A	0.563	0.558	G	0.437	0.442	0.812	0.817	1.000	1	0.980	0.619–1.551	0.884	1

CI, confidence interval; freq, frequency; HWE, Hardy–Weinberg equilibrium; OR, odds ratio; SNP, single-nucleotide polymorphism; SPS, sticky platelet syndrome.

Table 4. Frequency of haplotypes of the GP6 SNPs (10 most frequent shown) in SPS patients and the control group

Haplotype	Freq (SPS)	Freq (controls)	Chi-squared (X2)	Chi-squared P	Chi-squared P (Bonferroni)	OR	CI (95%)
TTGTGA	0.228	0.174	1.444	0.229	1	1.421	0.799–2.526
CTGCAG	0.211	0.217	0.009	0.926	1	0.974	0.556–1.703
TTGTAA	0.173	0.192	0.146	0.702	1	0.891	0.491–1.613
CTGTGA	0.101	0.111	0.058	0.809	1	0.912	0.433–1.921
TTGTAG	0.093	0.105	0.088	0.766	1	0.890	0.413–1.917
CGATAG	0.058	0.077	0.403	0.525	1	0.743	0.295–1.864
CTGCAA	0.033	0.061	1.163	0.281	1	0.542	0.174–1.676
CGATAA	0.026	0.006	1.813	0.178	1	4.117	0.443–38.25
TTGTGG	0.018	0.009	0.508	0.476	1	2.107	0.259–17.12
TTATAG	0.013	0.012	0.008	0.928	1	1.097	0.145–8.284

CI, confidence interval; Freq, frequency; OR, odds ratio; SNP, single-nucleotide polymorphism; SPS, sticky platelet syndrome.

Table 5. Frequency of alleles of the GP6 SNPs in patients with SPS type I and the control group

SNP	Minor allele			Fisher's exact P (allelic)	Fisher's exact P (Bonferroni)	OR (minor allele)	CI (95%)	Fisher's exact P (genotypic)	Fisher's exact P (Bonferroni)
	Allele	Freq (SPS type I)	Freq (controls)
rs1654410	C	0.353	0.494	0.184	1	0.560	0.258–1.210	0.161	1
rs1671153	G	0	0.097	0.077	0.540	–	–	0.065	0.452
rs1654419	A	0	0.097	0.077	0.540	–	–	0.065	0.452
rs11669150	C	0.206	0.279	0.520	1	0.669	0.271–1.650	0.635	1
rs1613662	G	0	0.078	0.128	0.898	–	–	0.115	0.806
rs12610286	G	0.529	0.318	0.029	0.201	2.411	1.134–5.123	0.028	0.194
rs1654431	G	0.412	0.442	0.849	1	0.885	0.416–1.880	0.461	1

CI, confidence interval; freq, frequency; OR, odds ratio; SNP, single-nucleotide polymorphism; SPS, sticky platelet syndrome.

Table 6. Frequency of haplotypes of the GP6 SNPs (10 most frequent shown) in patients with SPS type I

Haplotype	Freq (SPS type I)	Freq (controls)	Chi-squared (X2)	Chi-squared P	OR	CI (95%)
TTGTGA	0.375	0.177	6.305	0.012*	2.749	1.223–6.174
CTGCAG	0.203	0.213	0.026	0.873	0.927	0.369–2.328
TTGTAA	0.124	0.194	0.978	0.323	0.578	0.193–1.730
TTGTAG	0.111	0.101	0.025	0.875	1.100	0.334–3.617
CTGTGA	0.087	0.108	0.148	0.700	0.776	0.212–2.833
TTGTGG	0.036	0.009	1.511	0.219	4.231	0.349–51.27
CTGTGG	0.030	0.010	0.783	0.376	2.939	0.241–35.82
CTGTAG	0.029	0.010	0.776	0.378	2.981	0.233–38.10
CTGCAA	0.001	0.065	2.290	0.130	0.014	2.991–649.9
CGATAG	0	0.084	3.122	0.077

CI, confidence interval; Freq, frequency; OR, odds ratio; SNP, single-nucleotide polymorphism; SPS, sticky platelet syndrome.

*Statistically significant (P lower than 0.05).

Mammen EF. Sticky platelet syndrome. Semin Thromb Hemost. 1999;25(2):361–5.

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Bick RL. Sticky platelet syndrome: a common cause of unexplained arterial and venous thrombosis. Clin Appl Thromb Hemost. 1998;4(2):77–81.

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