Aminoguanidine and N-acetyl-cysteine supress oxidative and nitrosative stress in EAE rat brains

Figures & data

Figure 1. The clinical score of neurological disorder (1–6) of rats after 7 and 15 days. CG – control group; EAE – rats with experimental autoimmune encephalomyelitis; CFA – rats treated with complete Freund's adjuvant; EAE + AG – EAE rats treated with aminoguanidine; AG – rats treated with aminoguanidine; EAE + NAC – EAE rats treated with N-acetyl-l-cysteine; NAC – rats treated with N-acetyl-l-cysteine. 1 – healthy rats; 2 – loss of tail tone; 3 – hindlimb weakness; 4 – hindlimb paralysis; 5 – hindlimb paralysis plus forelimb weakness; 6 – moribund or dead.

Figure 2. NO₂ and NO₃ concentration (nmol/mg prot.) in the rat WEM. CG – control group; EAE – rats with experimental autoimmune encephalomyelitis; CFA – rats treated with complete Freund's adjuvant; EAE + AG – EAE rats treated with aminoguanidine; AG – rats treated with aminoguanidine; EAE + NAC – EAE rats treated with N-acetyl-l-cysteine; NAC – rats treated with N-acetyl-l-cysteine.

Figure 3. MDA concentration (nmol/mg prot.) in the rat WEM. CG – control group; EAE – rats with experimental autoimmune encephalomyelitis; CFA – rats treated with complete Freund's adjuvant; EAE + AG – EAE rats treated with aminoguanidine; AG – rats treated with aminoguanidine; EAE + NAC – EAE rats treated with N-acetyl-l-cysteine; NAC – rats treated with N-acetyl-l-cysteine.

Figure 4. GSH concentration (nmol/mg prot.) in the rat WEM. CG – control group; EAE – rats with experimental autoimmune encephalomyelitis; CFA – rats treated with complete Freund's adjuvant; EAE + AG – EAE rats treated with aminoguanidine; AG – rats treated with aminoguanidine; EAE + NAC – EAE rats treated with N-acetyl-l-cysteine; NAC – rats treated with N-acetyl-l-cysteine.

Figure 5. SOD activity (U/mg prot.) in the rat WEM. CG – control group; EAE – rats with experimental autoimmune encephalomyelitis; CFA – rats treated with complete Freund's adjuvant; EAE + AG – EAE rats treated with aminoguanidine; AG – rats treated with aminoguanidine; EAE + NAC – EAE rats treated with N-acetyl-l-cysteine; NAC – rats treated with N-acetyl-l-cysteine.