Protective effect of polysaccharide-protein complex from a polypore mushroom, Phellinus rimosus against radiation-induced oxidative stress

Figures & data

Figure 1. Effect of PPC-Pr on gamma radiation-induced DNA damage on human blood leukocytes, estimated by comet aasay in terms of tail length (A), % DNA in tail (B), tail moment (C), olive tail moment (D). Column 1:0 Gy; 2: 4 Gy; 3; PPC-Pr 50 µg + 4 Gy; 4: PPC-Pr 100 µg + 4 Gy.

Figure 2. Effect of administration of PPC-Pr on tissue GSH levels of irradiated mice. Values are mean ± SD, n = 6, ***P < 0.001, *P < 0.05 compared to irradiated control (Bonferroni test); a, denotes P < 0.001 compared to normal (Bonferroni test).

Figure 3. Effect of administration of PPC-Pr on GR activity in the serum of irradiated mice. Values are mean ± SD, n = 6, ***P < 0.001 compared to irradiated control; a, denotes P < 0.001 compared to normal (Bonferroni test).

Table 1. Effect of administration of PPC-Pr on antioxidant enzyme activity in liver and brain tissues of irradiated mice (4 Gy)

Enzyme activity	Organ	0 Gy	4 Gy	Amifostine + 4 Gy	PPC-Pr + 4 Gy (5 mg/kg bwt)	PPC-Pr + 4 Gy (10 mg/kg bwt)
GPx (U/mg protein)	Liver	8.90 ± 0.65	5.80 ± 0.65^a	6.30 ± 0.40^ns	6.30 ± 0.40^ns	9.03 ± 0.92*
	Brain	6.70 ± 0.40	2.97 ± 0.31^a	5.90 ± 0.83^ns	6.25 ± 0.23**	6.40 ± 0.60**
SOD (U/mg protein)	Liver	5.80 ± 0.28	2.06 ± 0.24^a	2.94 ± 0.28***	3.27 ± 0.38***	4.55 ± 0.62***
	Brain	3.50 ± 0.24	1.08 ± 0.10^a	1.50 ± 0.14^ns	1.75 ± 0.16***	2.96 ± 0.20***
CAT (U/mg protein)	Liver	19.76 ± 2.00	10.76 ± 1.20^a	16.95 ± 1.50***	12.5 ± 5.66***	17.66 ± 2.10***
	Brain	12.76 ± 1.10	5.20 ± 0.57^a	6.08 ± 0.36^ns	5.66 ± 0.48***	10.88 ± 0.89***

Values are mean ± SD, n = 6, ***P < 0.001, **P < 0.01, *P < 0.05; ns, non significant compared to irradiated control (Bonferroni test); a, denotes P < 0.001 compared to normal (Bonferroni test).

Figure 4. Effect of administration of PPC-Pr on tissue MDA levels of irradiated mice. Values are mean ± SD, n = 6, ***P < 0.001; ns, non significant compared to irradiated control; a, denotes P < 0.001 compared to normal (Bonferroni test).

Figure 5. Effect of administration of PPC-Pr on survival rate of animals exposed to 9 Gy gamma radiation.