The flavonoid morin restores blood pressure and lipid metabolism in DOCA-salt hypertensive rats

Figures & data

Figure 1. Effect of morin on MAP (A), heart rate (B), and body weight (C) in UNX and DOCA-salt hypertensive rats. Values are expressed as means ± standard deviation (SD) for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT).

Table 1. Effect of morin on TC and TG in plasma and tissues of UNX and DOCA-salt hypertensive rats

Parameter		UNX	UNX + morin	DOCA-salt	DOCA-salt + morin
TC (mg/g tissue)	Plasma (mg/dl)	81.13 ± 6.41^a	81.76 ± 5.8 3^a	163.06 ± 11.24^b	94.13 ± 5.43^c
	Kidney	4.16 ± 0.28^a	4.03 ± 0.19^ac	6.93 ± 0.46^b	4.52 ± 0.39^c
	Heart	2.49 ± 0.15^a	2.38 ± 0.17^a	4.53 ± 0.24^b	2.86 ± 0.21^c
	Aorta	3.03 ± 0.23^a	2.98 ± 0.16^a	6.82 ± 0.43^b	4.12 ± 0.27^c
	Liver	4.51 ± 0.33^a	4.48 ± 0.31^a	7.36 ± 0.53^b	5.03 ± 0.41^c
TG (mg/g tissue)	Plasma (mg/dl)	56.17 ± 3.87^a	55.11 ± 3.97^a	158.16 ± 9.33^b	69.66 ± 5.65^c
	Kidney	4.31 ± 0.29^a	4.17 ± 0.23^a	7.01 ± 0.59^b	4.86 ± 0.26^c
	Heart	3.96 ± 0.21^a	3.99 ± 0.26^a	5.83 ± 0.32^b	4.07 ± 0.29^c
	Aorta	2.89 ± 0.19^a	2.83 ± 0.22^a	6.24 ± 0.46^b	4.02 ± 0.26^c
	Liver	3.72 ± 0.26^a	3.53 ± 0.28^a	7.61 ± 0.43^b	3.94 ± 0.32^c

Values are expressed as means ± SD for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT).

Table 2. Effect of morin on FFA and PL in plasma and tissues of UNX and DOCA-salt hypertensive rats

Parameter		UNX	UNX + morin	DOCA-salt	DOCA-salt + morin
FFA (mg/g tissue)	Plasma (mg/dl)	53.91 ± 3.66^a	54.03 ± 4.26^a	121.66 ± 9.55^b	66.21 ± 5.34^c
	Kidney	4.23 ± 0.26^a	4.18 ± 0.32^a	7.96 ± 0.45^b	5.03 ± 0.36^c
	Heart	4.96 ± 0.27^a	4.77 ± 0.29^a	9.13 ± 0.74^b	6.12 ± 0.42^c
	Aorta	3.92 ± 0.31^a	3.92 ± 0.26^a	7.26 ± 0.52^b	5.02 ± 0.39^c
	Liver	7.73 ± 0.36^a	7.56 ± 0.61^a	16.86 ± 1.16^b	9.14 ± 0.63^c
PL (mg/g tissue)	Plasma (mg/dl)	96.43 ± 6.62^a	94.16 ± 7.61^ac	156.13 ± 8.11^b	104.11 ± 7.38^c
	Kidney	15.76 ± 0.99^a	14.63 ± 1.02^a	28.66 ± 1.98^b	19.03 ± 1.31^c
	Heart	13.18 ± 0.76^a	12.97 ± 0.98^a	23.14 ± 1.58^b	15.43 ± 1.23^c
	Aorta	4.73 ± 0.25^a	4.52 ± 0.19^a	10.66 ± 0.82^b	6.26 ± 0.57^c
	Liver	21.16 ± 1.51^a	21.32 ± 1.72^a	53.23 ± 3.76^b	28.31 ± 1.82^c

Values are expressed as means ± SD for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT).

Figure 2. Effect of morin on LDL-C, VLDL-C, and HDL-C in plasma of UNX and DOCA-salt hypertensive rats. Values are expressed as means ± SD for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT).

Figure 3. Effect of morin on the activity of HMG-CoA reductase in plasma and tissues of UNX and DOCA-salt hypertensive rats. Values are expressed as means ± SD for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT). *Lower ratio indicates higher enzyme activity.

Figure 4. Effect of morin on the activity of LCAT in plasma of UNX and DOCA-salt hypertensive rats. Values are expressed as means ± SD for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT).

Figure 5. Effect of morin on the levels of urinary protein in UNX and DOCA-salt hypertensive rats. Values are expressed as means ± SD for six rats in each group. Values not sharing a common superscript differ significantly at P < 0.05 (DMRT).

Figure 6. Photomicrograph of liver section staining with H & E (A–F; 10×) and MT (G–L; 10×). UNX-control (A and G), UNX-control treated with morin alone (50 mg/kg) (B and H), DOCA-salt hypertensive rats (C–E and I–K) and DOCA-salt rats treated with morin (50 mg/kg) (F and L). Scale bar: 5 µm.

Table 3. Effect of morin on the degree of histological changes of liver in UNX and DOCA-salt hypertensive rats

Groups	UNX	UNX + morin	DOCA-salt	DOCA-salt + morin
H&E staining
Sinusoidal dilatation	A	A	++ +	+
Kuffer cell hyperplasia	A	A	++ +	A
Congested blood vessels	A	A	++ +	A
Necrosis with fatty deposits	A	A	++ +	A
MT staining
Collagen deposition	A	A	++ +	A

Photomicrographs were used to evaluate the degree of damages in the liver tissues. (A) no changes; (+) mild changes; (++ ) moderate changes; (+++) marked changes.