Routine application of a novel MLPA-based first-line screening test uncovers clinically relevant copy number aberrations in haematological malignancies undetectable by conventional cytogenetics

Figures & data

Table 1. Cases studied according to primary clinical indication

Table 2. Genomic regions interrogated by new MLPA probemix P377

Table 3. Genomic aberrations detected by the new MLPA probemix, all with normal karyotype or unrelated karyotypic abnormalities

Döhner H, Stilgenbauer S, Benner A, Leupolt E, Kröber A, Bullinger L, et al. Genomic aberrations and survival in chronic lymphocytic leukemia. N Engl J Med. 2000;343:1910–6.

 PubMed Web of Science ®Google Scholar

Stevens-Kroef M, Simons A, Gorissen H, Feuth T, Weghuis DO, Buijs A, et al. Identification of chromosomal abnormalities relevant to prognosis in chronic lymphocytic leukemia using multiplex ligation-dependent probe amplification. Cancer Genet Cytogenet. 2009;195:97–104.

 PubMed Web of Science ®Google Scholar

Van Bockstaele F, Verhasselt B, Philippe J. Prognostic markers in chronic lymphocytic leukaemia: a comprehensive review. Blood Rev. 2009;23:25–47.

 PubMed Web of Science ®Google Scholar

Shao L, Kang SH, Li J, Hixson P, Taylor J, Yatsenko SA, et al. Array comparative genomic hybridization detects chromosomal abnormalities in hematological cancers that are not detected by conventional cytogenetics. J Mol Diagnost. 2010;12:670–8.

 PubMed Web of Science ®Google Scholar

Haferlach T, Kern W, Schnittger S, Schoch C. Modern diagnostics in acute leukemias. Crit Rev Oncol Hematol. 2005;56:223–34.

 PubMed Web of Science ®Google Scholar

Mullighan CG, Miller CB, Radtke I, Phillips LA, Dalton J, Ma J, et al. BCR-ABL1 lymphoblastic leukaemia is characterized by the deletion of Ikaros. Nature. 2008;453:110–4.

 PubMed Web of Science ®Google Scholar