Water influx into cerebrospinal fluid is significantly reduced in senile plaque bearing transgenic mice, supporting beta-amyloid clearance hypothesis of Alzheimer’s disease

Figures & data

Figure 1. Regions of interests (ROIs) were selected semi-automatically using image processing software. BG: basal ganglia, CSF: cerebrospinal fluid.

Figure 2. Decay curve fitting. Intensities at the steady state of each area, expressed as% against the averaged intensity of identical pixel prior to administration of H₂¹⁷O, were determined by fitting their time course using the function: I  =  I₀(1 − ae^−bt). I₀ denotes the normalized signal intensity at infinite time (t  =  ∞) calculated from the fitted curve.

Figure 3. Representative time course. Representative time course of signal intensities within pixels of each region of interest (ROI) shown in Fig. 1 following intravenous H₂¹⁷O administration in transgenic (APP-PS1) and age-matched wild-type (WT) mice. Blue: cortex, red: basal ganglia (BG), green: cerebrospinal fluid (CSF) within the third ventricle. Each dot represents the intensity of each pixel within the ROI.

Figure 4. I₀ of three ROIs. Values of I₀ in cortex and basal ganglia (BG) are virtually identical between APP-PS1 and WT mice. In contrast, I₀ of CSF within the third ventricle is significantly higher in APP-PS1 mice. For comparison, previously reported values of aquaporin-4 (AQP-4) knock out mice are shown together. *P < 0·05. WT, wild type; APP-PS1, APP-PS1 transgenic; AQP4(−/−), AQP4 knockout.

Figure 5. Beta-amyloid immunohistology of APP-PS1 transgenic (left) and control (right) mice.