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Abstract
In the present work, a nanocomposite scaffold was prepared on the basis of collagen, hyaluronic acid (HA) and nanobioactive glass (NBAG) by the freeze drying method for the application to tissue engineering scaffold materials. The biological property assays, including von Kossa staining, tetracycline staining, hemolysis, platelet adhesion test, pyrogen tests and acute toxicity test, were performed according to the requirements of ISO 10993 standards to evaluate its performance as applicable tissue engineering. The results indicated that the collagen-HA/NBAG scaffold possessed a homogeneous morphology and interconnected pore structure, with pore sizes ranging from 50 to 500 μm in diameter. Examination of the hemolytic potential showed that the nanocomposite scaffolds were non-hemolytic in nature and showed blood compatibility. The platelet adhesion tests showed that the collagen-HA/NBAG material had an excellent anticoagulation property. The collagen-HA/NBAG scaffolds showed no acute systemic toxicity or pyrogen reaction. The results of von Kossa and tetracycline stainings indicated that the NBAG can promote fibroblastic differentiation and improve the mineral deposition of the collagen/HA. Therefore, the collagen-HA/NBAG composite materials would be a promising candidate of biomedical materials for tissue engineering.
Acknowledgements
This work was financially supported by the National Natural Science Fund of China [grant number 51273062], [grant number 51443002]; the Hunan Provincial Natural Science Fund of China [grant number 12JJA003] and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.