Detection of Uncured Visible Implant Elastomer Tags in Larval Pacific Lampreys

Abstract

Lampreys in the Columbia River basin are a conservation concern, and understanding their status is a priority among managers. Many population assessment techniques depend on the ability to tag fish so that these tags are retained for the duration of the study with minimal effect on survival. There are several methods for tagging small fishes in streams, including passive integrated transponders, coded wire tags, and visible implant elastomer (VIE) tags. Of these, VIE tags have not been extensively tested on larval or juvenile lampreys. We evaluated the performance of uncured VIE tags on ammocoetes of Pacific lampreys Lampetra tridentata in a laboratory experiment to determine tag detection and the influences of color and tag position on detection. Additionally, we examined the effects of transformation (ammocoete to macrophthalmia) on tag detection. Though survival was not specifically tested, 0 of the 84 lampreys died as a result of tagging. Tag detection was 87% after 168 d in lampreys that did not transform; in those that did transform, however, tag detection was reduced to 38%. Tag position did not affect detection, but red and orange tags were detected more consistently than green tags. Overall, uncured VIE tags can be used effectively to tag larval lampreys; however, studies that depend on long-term tag detection either should be conducted at a time when lampreys are not expected to transform or should only use ammocoetes that are not likely to transform during the study.