Abstract
An oxytetracycline hydrochloride (OTC) marking study was used to determine an OTC concentration that would successfully mark palmetto bass (male white bass Morone chrysops × female striped bass M. saxatilis) in hard hatchery water (hardness as CaCO3 = 500 mg/L). Outcomes for juvenile palmetto bass immersed for 6 h in 500- and 700-mg/L concentrations of OTC mixed with hard water were compared to determine whether 14-d marking mortality and mark intensity at 14 and 344 d postimmersion differed between immersion concentrations. Sagittal otoliths were examined for marks at 14 d; lapillus otoliths were examined for marks at 344 d. Marking mortality was not detectable after 14 d, but identification and intensity of sagittal otolith marks at 14 d postimmersion were poor for both OTC concentrations. Oxytetracycline marks were identified in 63% (500 mg/L) and 74% (700 mg/L) of the sagittae examined at 14-d postimmersion, whereas marks were found in 87% (500 mg/L) and 95% (700 mg/L) of lapilli examined at 344 d postimmersion. The 700-mg/L OTC concentration yielded better mark intensity results than the 500-mg/L concentration in lapilli at 344 d postimmersion.