Online coupling of capillary electrophoresis with mass spectrometry for the identification of biomarkers for clinical diagnosis

Abstract

Proteomic screening of complex biologic samples is of increasing importance in clinical research and diagnosis. In the postgenomic area it is evident that changes of the composition of body fluids, as well as post-translational modifications of proteins and peptides, provide more information than genetic typing. The study of these changes allows the state of health or disease of particular organs, and consequently, the whole organism, to be described. This review describes the application of capillary electrophoresis coupled online to an electrospray ionization time-of-flight mass spectrometer to the analysis of body fluids obtained from patients for the identification of biomarkers for diagnostic purposes.

Keywords::

• biomarker
• capillary electrophoresis
• clinical diagnosis
• mass spectrometry
• proteomics