Influence of matrix application timing on spectral reproducibility and quality in SELDI-TOF-MS

Figures & data

Table 1.  Summary of Peak Area Comparisons

ProteinChip Array	Binding Buffer	Matrix	Drying Time-Dependent Differences Drying Time (min)
			1	2	3	5	8	10
H4	25% ACN	CHCA	=	=	=	−	−	−
H4	25% ACN	SPA	=	=	=	=	=	=
H50	25% ACN, 0.1% TFA	CHCA	=	=	=	=	=	=
H50	25% ACN, 0.1% TFA	SPA	=	=	=	=	=	=
IMAC3	1× PBS, pH 7.4	CHCA	=	=	=	=	=	=
IMAC3	1× PBS, pH 7.4	SPA	=	=	=	=	=	=
SAX2	50 mM Tris-HCl, pH 8.0	CHCA	=	=	=	=	=	=
SAX2	50 mM Tris-HCl, pH 8.0	SPA	=	=	=	=	=	=
WCX2	50 mM Tris-HCl, pH 8.0	CHCA	+	+	+	+	+	+
WCX2	50 mM Tris-HCl, pH 8.0	SPA	=	=	=	=	=	=
IMAC40	1× PBS, pH 7.4	CHCA	=	=	=	=	=	=
IMAC40	1× PBS, pH 7.4	SPA	=	=	=	=	=	=
LSAX30	50 mM Tris-HCl, pH 8.0	CHCA	=	=	=	=	−	−
LSAX30	50 mM Tris-HCl, pH 8.0	SPA	=	=	=	=	=	=
LWCX30	50 mM Tris-HCl, pH 8.0	CHCA	=	=	=	=	=	=
LWCX30	50 mM Tris-HCl, pH 8.0	SPA	=	=	=	=	=	=

Statistically significant (P < 0.01) decreases (−), increases (+), or no change (=) in mean peak area between control (0) and each drying time are indicated. ACN, acetonitrile; CHCA, α-cyano-4-hydroxy cinnamic acid; SPA, sinapinic acid; TFA, trifluoracetic acid; PBS, phosphate-buffered saline.

Figure 1.  LSAX30 ProteinChip array data summary.

(A) Representative unnormalized and normalized spectra at time point 0 min and time point 10 min. (B) Expanded view of normalized spectra showing peaks of interest at time point 0 min and time point 10 min. (C) Mean peak area (± sd) representation for two peaks of interest at each drying time. (D) Mean signal-to-noise ratio (± sd) representation for two peaks of interest at each drying time.