Cleaning Microsatellite PCR Products with Sephadex™ in 96-Well Filtration Plates Enhances Genotyping Quality

Figures & data

Figure 1. Effect of Sephadex filtration on the quality of gel-based analysis of microsatellite PCR products.

Shown are two 96-lane gels of the same microsatellite reactions (A) without filtration and (B) following Sephadex filtration. Each lane represents a multiplex of six microsatellite loci. Amplifications were performed as described in Reference (3) using DNA from collared lizards (Crotaphytus collaris).

Hutchison, D.W., J.L.Strasburg, J.A.Brisson, and S.Cummings. Isolation and characterization of eleven polymorphic loci in collared lizards (Crotaphytus collaris). Mol. Ecol. Notes In press.

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