Stem Cells Out of the Bag: Characterization of Ex Vivo Expanded Mesenchymal Stromal Cells for Possible Clinical Use

Figures & data

Table 1. Mononuclear cell recovery and viability for each donor.

Sample	Age	Sex	Recovered MNC × 10^6		Estimated BM volume (ml)	Recovered MNC viability (%)
			Bag/filter	Filtered BM sample
BM1	37	F	43.9	1.4	31	94
BM2	31	F	201.3	4.5	44	96
BM3	32	F	103.3	1.5	69	93
BM4	24	F	662	3.7	18	97
BM5	41	M	78.6	1.2	68	96
BM6	33	F	238.5	15.4	39	95
BM7	28	F	119.2	1.6	76	98
BM8	42	F	180.0	2.8	65	96
BM9	43	F	66.3	1.1	61	94
BM10	31	F	123.4	1.4	87	95
BM11	23	M	140.9	2.4	58	93
BM12	27	M	215.7	3.0	71	92
BM13	42	M	55.4	2.2	25	99
BM14	25	F	61.4	0.8	58	96
BM15	22	M	181.5	11.7	12	94
BM16	45	M	54.4	4.4	15	95
BM17	48	F	165.5	4.5	48	92
BM18	35	M	249.0	1.9	49	96
BM19	51	M	645.2	4.2	214	94
BM20	26	F	373.0	4.7	84	93

Comparison of number of mononuclear cell isolated from the filtered bone marrow sample with the correspondent bag/filter washing.

BM: Bone marrow; MNC: Mononuclear cell.

Figure 1. Histograms of the immunophenotype assessed by flow cytometry.

Example of one representative culture of mesenchymal stromal cells.

Table 2. Immunophenotype analysis of mesenchymal stromal cells obtained, for each surface antigen evaluated and standard deviation.

Surface antigen	Average positive cells (%)
CD3	1 ± 0.8
CD14	3 ± 1.9
CD34	0 ± 0.8
CD45	3 ± 1.4
CD44	96 ± 3.6
CD73	95 ± 3.5
CD90	96 ± 3.1
CD105	96 ± 3.5

Figure 2. CFSE histograms.

Proliferating responder cells are depicted in light gray, in the absence (A) and presence (B) of mesenchymal stromal cells. Dark gray represents nonproliferating cells.

Figure 3. Mesenchymal stromal cell adipogenic differentiation.

(A) Negative control; (B) mesenchymal stromal cell-derived adypocytes with oil red O-stained droplets evident.