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Future Science OA Volume 8, 2022 - Issue 4
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Short Communication

Hyperacute Transplantation of Umbilical Cord Mesenchymal Stromal Cells in a Model of Severe Intracerebral Hemorrhage

Tanira Giara Mello1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil;2 Instituto de Engenharia Nuclear, Comissão Nacional de Energia Nuclear, Rio de Janeiro, RJ, 21941-614, Brazil;3 Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, RJ, 21941-902, Brazil
,
Paulo Henrique Rosado-de-Castro3 Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, RJ, 21941-902, Brazil;4 Departamento de Radiologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil;5 Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, BrazilORCID Iconhttps://orcid.org/0000-0003-0091-9714
ORCID Icon,
Juliana Ferreira Vasques1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil;3 Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, RJ, 21941-902, Brazil;5 Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, BrazilORCID Iconhttps://orcid.org/0000-0003-1581-670X
ORCID Icon,
Carolina Pinhão1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, BrazilORCID Iconhttps://orcid.org/0000-0001-6770-8730
ORCID Icon,
Tayná Monteiro Santos1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, BrazilORCID Iconhttps://orcid.org/0000-0002-4708-7698
ORCID Icon,
Renata Rodrigues de Lima1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil
,
Bernd Uwe Foerster6 Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, 13566-590, Brazil
,
Fernando Fernandes Paiva6 Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, 13566-590, Brazil
,
Rosalia Mendez-Otero1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil;3 Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, RJ, 21941-902, BrazilORCID Iconhttps://orcid.org/0000-0002-1798-9930
ORCID Icon &
Pedro Moreno Pimentel-Coelho1 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil;3 Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, RJ, 21941-902, BrazilCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-2414-3386
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Article: FSO793 | Received 05 Oct 2021, Accepted 24 Feb 2022, Published online: 24 Mar 2022

Figures & data

Figure 1. Experimental design.

hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage; iv.: Intravenous; MRI: Magnetic resonance imaging.

Figure 1. Experimental design.hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage; iv.: Intravenous; MRI: Magnetic resonance imaging.
Figure 2. Survival analysis.

Animals were treated with (ICH + hUC-MSC) or with the vehicle (ICH + vehicle) 1 h after the induction of ICH. The graph shows the survival curves during the 22 days of follow-up. Log-rank test (Mantel–Cox test) was used. n = 15 (ICH + vehicle) and n = 16 (ICH + hUC-MSC) rats per group.

hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage.

Figure 2. Survival analysis.Animals were treated with (ICH + hUC-MSC) or with the vehicle (ICH + vehicle) 1 h after the induction of ICH. The graph shows the survival curves during the 22 days of follow-up. Log-rank test (Mantel–Cox test) was used. n = 15 (ICH + vehicle) and n = 16 (ICH + hUC-MSC) rats per group.hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage.
Figure 3. Motor function assessment.

Animals were treated with (ICH + hUC-MSC) or with the vehicle (ICH + vehicle) 1 h after the induction of ICH. The graph shows the latencies to fall in the rotarod test at different time points from surgery. Mixed-effects analysis and Sidák’s post-hoc test were used. Data shown in the graph are means ± SD; n = 12-13 animals in the ICH + vehicle group, n = 14 animals in the ICH + hUC-MSC group.

hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage; SD: Standard deviation.

Figure 3. Motor function assessment.Animals were treated with (ICH + hUC-MSC) or with the vehicle (ICH + vehicle) 1 h after the induction of ICH. The graph shows the latencies to fall in the rotarod test at different time points from surgery. Mixed-effects analysis and Sidák’s post-hoc test were used. Data shown in the graph are means ± SD; n = 12-13 animals in the ICH + vehicle group, n = 14 animals in the ICH + hUC-MSC group.hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage; SD: Standard deviation.
Figure 4. Evaluation of the residual hematoma volume.

Animals were treated with (ICH + hUC-MSC) or with the vehicle (ICH + vehicle) 1 h after the induction of ICH. (A) Representative T1-weighted images obtained with a 3D sequence in a 2 Tesla MRI scanner 22 days after the induction of ICH. (B) Graph showing the quantification of the residual hematoma volume. Data shown in the graphs are individual values, and means ± SD Mann–Whitney U test; n = 11 (ICH + vehicle) and n = 14 (ICH + hUC-MSC) rats per group.

hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage.

Figure 4. Evaluation of the residual hematoma volume.Animals were treated with (ICH + hUC-MSC) or with the vehicle (ICH + vehicle) 1 h after the induction of ICH. (A) Representative T1-weighted images obtained with a 3D sequence in a 2 Tesla MRI scanner 22 days after the induction of ICH. (B) Graph showing the quantification of the residual hematoma volume. Data shown in the graphs are individual values, and means ± SD Mann–Whitney U test; n = 11 (ICH + vehicle) and n = 14 (ICH + hUC-MSC) rats per group.hUC-MSC: Human umbilical cord-derived mesenchymal stromal cells; ICH: Intracerebral hemorrhage.

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