Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs

Figures & data

Table 1. Primers sequences.

Gene	Forward (5′-3′)	Reverse (5′-3′)
ANGPTL4	CCTCTCCGTACCCTTCTCCA	AAACCACCAGCCTCCAGAGA
NF-κB1	TGGGAAGGCCTGAACAAATG	TATGGGCCATCTGTTGGCAG
Notch1	CTGAATTTCACTGTGGGCGG	CCCCGCAGAGGGTTGTATTG
18s rRNA	AGGAATTCCCAGTAAGTGCG	GCCTC ACTAAACCATCCAA

Table 2. siRNA sequences.

Gene	Sense	Anti-sense
ANGPTL4	GGUCUGGAGAAGGUGCAUAtt	UAUGCACCUUCUCCAGACCca
NF-κB1	GGAUUUCGUUUCCGUUAUGtt	CAUAACGGAACGAAAUCCtc
Notch1	GGGAGCAUGUGUAACAUCAtt	UGAUGUUACACAUGCUCCCtg
Scrambled	UUCUCCGAACGUGUCACGU	ACGUGACACGUUCGGAGAA

Figure 1. The IC₅₀ of gemcitabine on PANC-1 cell line.

n = 3.

Figure 2. Interactions and silencing efficacy of the targeted genes.

(A) Protein–protein interaction using STRING database, the PPI enrichment p-value: 0.0681. (B) Relative normalized expression of Notch1 siRNA, ANGPTL4 siRNA, NF-κβ1 siRNA in cells transfected with 100 nM siRNA targeting each gene's mRNA. The results normalized to scrambled-siRNA.

n = 3, values are (mean ± SD).

Figure 3. The dose-dependent assay for siRNAs (Notch1, scrambled (SC), ANGPLT4 and NF-κβ1) from left to right, respectively.

siRNAs compared with mocked transfected PANC-1 cell line. Significant differences compared with SC siRNA.

***p < 0.001; ****p < 0.0001.

n = 3, values are (mean ± SD); ns: Not significant.

Figure 4. (100 nM) of each single siRNA with 6 μM gemcitabine compared with mocked transfected PANC-1 cell line.

n = 3, values are (mean ± SD). Significant differences compared with SC siRNA + Gemcitabin. Statistical significance: **** indicates p < 0.0001.

Figure 5. NF-κβ1, Notch1, ANGPTL4 (100 nM) single combinations with 1:1 molar ratio with and without 6 μM gemcitabine compared with mocked transfected PANC-1 cell line.

Significant differences compared with SC siRNA.

****p < 0.0001.

n = 3, values are (mean ± SD).