Arthritis alleviation: unveiling the potential in Abrus precatorius macerated oil

Figures & data

Figure 1. Abrus precatorius plant.

Table 1. Preliminary phytochemical screening tests of Abrus precatorius macerated oil.

S.No.	Name of the sample	Phytochemical compound	Result
1.	Macerated oil	Resins	+
2.		Carboxylic acid	–
3.		Tanins	–
4.		Steroids	–
5.		Flavonoid	+
6.		Carbohydrates	+
7.		Glycosides	–
8.		Saponification	–
9.		Protein	–
10.		Phenol	–
11.		Biuret	–
12.		Saponin	+
13.		Gum	+
14.		Flavanoglycosides	–
15.		Alkaloids	+

+ Indicated presence and – indicates absence.

Figure 2. Calibration curve of gallic acid at various concentrations.

Figure 3. Calibration curve of tannic acid at various concentrations.

Figure 4. Calibration curve of cortisone acetate at various concentrations.

Figure 5. DPPH scavenging activity.

Figure 6. ABTS scavenging activity.

Figure 7. Ferric reducing activity of macerated oil.

Figure 8. Nitric oxide scavenging activity.

Figure 9. Albumin denaturation assay.

Figure 10. Proteinase inhibition assay.

Table 2. Phytoconstituents detected in macerated oil using gas chromatography and mass-spectroscopy.

S.NO	Retention time	Compound name	Molecular weight	Molecular formula
1	14.43	N-Hexadecenoic acid	256	C16H32O2
2	16.59	T-Butyl Cyclopentaneperoxycarboxylate	186	C10H18O3
3	25.20	Octadecane	254	C18H38
4	26.54	Octanoyl Chloride	162	C8H15OCl
5	29.68	Octanoic acid	316	C14H18O2BrF
6	30.19	Myristic anhydride	438	C16H30O2
7	32.40	Tetracosane	338	C24H50

Figure 11. Chromatogram of macerated oil using gas chromatography and mass spectroscopy.

Table 3. Molecular docking scores of bioactive compounds from Abrus precatorius macerated oil against TNF α and IL 6.

S.no	Ligands	Binding energy TNF- α (Kcal/mol)	Binding energy IL-6 (Kcal/mol)
1	T-Butyl cyclopentaneperoxycarboxylate	-7.5	-6.3
2	Octadecane	-6.5	-5.3
3	Octanoyl chloride	-6.6	-6.7
4	Octanoic acid	-7.7	-6.1
5	Myristic anhydride	-8.6	-5.6
6	Tetracosane	-6.9	-4.2