HMMR antisense RNA 1, a novel long noncoding RNA, regulates the progression of basal-like breast cancer cells

Figures & data

Table 1 Primers for real-time polymerase chain reaction analysis

Gene name	Forward	Reverse
β-Actin	5′-CCACTGGCATCGTGATGGA-3′	5′-CGCTCGGTGAGGATCTTCAT-3′
HMMR	5′-ATGATGGCTAAGCAAGAAGGC-3′	5′-TTTCCCTTGAGACTCTTCGAGA-3′
HMMR-AS1	5′-CACAACTCCTGGTTCCTG-3′	5′-GGATGGGTATTGGTCTGT-3′

Table 2 Sequences for small interfering RNA analysis

Gene name	Sense (5′–3′)	Antisense (5′–3′)
HMMR-si1	5′-CAAGAAUCAUGGAAGUAAACAUCTG-3′	5′-CAGAUGUUUACUUCCAUGAUUCUUGAC-3′
HMMR-AS1-si2	5′-GUAGAAGCCACUAGAACUAUCUGTA-3′	5′-UACAGAUAGUUCUAGUGGCUUCUACUU-3′

Figure 1 Expression levels of HMMR-AS1 (RP11-80G.1) long noncoding RNA were analyzed by RT-qPCR in 52 breast cancer tissue samples. (A) Expression levels of HMMR in breast cancer tissues were found by RT-qPCR to be significantly lower than those in adjacent normal tissues. (B) The expression levels of HMMR were significantly correlated with those of HMMR-AS1 (RP11-80G.1) lncRNA (R=0.802, P<0.0001). *Maximum value; □ mean value.

Abbreviations: HMMR-AS1, HMMR antisense RNA 1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; HMMR, hyaluronan-mediated motility receptor; lncRNA, noncoding RNA; BLBC, basal-like breast cancer.

Figure 2 The expression levels of HMMR mRNA and HMMR-AS1 long noncoding RNA in (A) MDA-MB-231 and (B) MDA-MB-468 cells after siRNA transfection. The results showed that the expression levels of HMMR-AS1 and HMMR were significantly reduced in HMMR-AS1 siRNA groups. *P<0.05 compared with the siRNA control group.

Abbreviations: HMMR, hyaluronan-mediated motility receptor; HMMR-AS1, HMMR antisense RNA1; siRNA, small interfering RNA; NC, negative control; mRNA, messenger RNA.

Figure 3 Effects of HMMR-AS1 knockdown on the proliferation of MDA-MB-231 and MDA-MB-468 cells. HMMR-AS1 siRNAs evidently inhibited the growth of (A) MDA-MB-231 and (B) MDA-MA-468 compared with NC siRNA in an MTS assay (*P<0.05).

Abbreviations: HMMR-AS1, HMMR antisense RNA1; siRNA, small interfering RNA; NC, negative control; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium.

Figure 4 Effects of HMMR-AS1 knockdown on the migration ability of MDA-MB-231 and MDA-MB-468 cells. The capacity of cell invasion in the (A) MDA-MB-231 and (B) MDA-MB-468 cells was apparently impaired by HMMR-AS1 siRNA. *P<0.05 compared with that of the NC siRNA control.

Abbreviations: HMMR-AS1, HMMR antisense RNA1; siRNA, small interfering RNA; NC, negative control.

Table 3 Correlations of HMMR-AS1 with other cancer-related genes

Gene name	Correlation coefficients with HMMR-AS1
BRCA1	R = 0.57, P<0.05
BRCA2	R = 0.62, P<0.05
PTEN	R = 0.62, P<0.05

Abbreviation: HMMR-AS1, hyaluronan-mediated motility receptor antisense RNA 1.