Expression and Significance of LINC02418 in Breast Cancer

Figures & data

Table 1 The Association of LINC02418 with patients’ clinicopathological features

Variant	Cases (n=129)	LINC02418 expression		P
		Low (n=61)	High (n=68)
Menopause				0.105
No	58	32	26
Yes	71	29	42
Age				0.095
<50	64	35	29
≥50	65	26	39
Smoking history				0.998
No	74	35	39
Yes	55	26	29
HER-2 status				0.052
Negative	52	30	22
Positive	77	31	46
PR status				0.612
Negative	77	35	42
Positive	52	26	26
ER status				0.108
Negative	73	30	43
Positive	56	31	25
Tumor size				0.956
<5	68	32	36
≥ 5	61	29	32
Differentiation				0.654
Well-moderate	82	40	42
Poor	47	21	26
TNM stage				0.003
I-II	82	47	35
III	47	14	33
Lymph node metastasis				0.007
No	84	47	37
Yes	45	14	31

Figure 1 LINC02418 expression was upregulated in breast cancer tissues than adjacent normal tissues (a). LINC02418 expression was upregulated in breast cancer cells than normal breast cells (MCF-10A) (b). Cox multifactorial analysis of independent factors affecting patient prognosis (c). LINC02418 high expression group with low survival at 5 years (d). ***p<0.001, compared with normal tissue and normal cells.

Figure 2 miR-766-5p expression was downregulated in breast cancer tissues than adjacent normal tissues (a) miR-766-5 expression was downregulated in breast cancer cells than normal breast cells (MCF-10A) (b). LINC02418 was negatively correlated with miR-766-5p (c). ***p<0.001, compared with normal tissue and normal cells.

Figure 3 miR-766-5p affects LINC02418 luciferase activity (a). si-LINC02418 effectively knocks down LINC02418 expression (b). Knockdown of LINC02418 reduces miR-766-5p expression (c). **p< 0.001, compared with control group. ^##p< 0.01 compared with the si-LINC02418 group.

Abbreviation: NC: negative control. si: silent, knockdown of LINC02418 expression.

Figure 4 Knockdown of LINC02418 inhibited cellular undesirable biological behavior, which was reversed by low expression of miR-766-5p (a-c). **p<0.001, compared with control group; ^##p< 0.01 compared with the si-LINC02418 group.