Figures & data
Figure 1 Schematic diagram showing the immobilization of herceptin on mercaptosuccinic acid-coated gold nanoparticles.
![Figure 1 Schematic diagram showing the immobilization of herceptin on mercaptosuccinic acid-coated gold nanoparticles.](/cms/asset/2cd54a4d-9fda-412d-ab40-f04b7c242635/dbct_a_25709498_f0001_c.jpg)
Figure 2 UV spectra of GNP and GNP–Her.
![Figure 2 UV spectra of GNP and GNP–Her.](/cms/asset/ba05d589-9f3d-41bd-968c-36a02a63f4e8/dbct_a_25709498_f0002_c.jpg)
Figure 3 FT-IR spectra of GNP (A) and GNP–Her (B) measured using the KBr method.
![Figure 3 FT-IR spectra of GNP (A) and GNP–Her (B) measured using the KBr method.](/cms/asset/1533844e-0553-4a95-9b06-054eac13f16f/dbct_a_25709498_f0003_c.jpg)
Figure 4 TEM images of GNP (A) and GNP–Her (B).
![Figure 4 TEM images of GNP (A) and GNP–Her (B).](/cms/asset/1d8f5a7b-9384-43a3-82f0-8e048b47e541/dbct_a_25709498_f0004_c.jpg)
Figure 5 Particle-size distributions of GNP (A) and GNP–Her (B) measured by DLS.
![Figure 5 Particle-size distributions of GNP (A) and GNP–Her (B) measured by DLS.](/cms/asset/b0a8baf6-0c59-494a-81aa-7a20958edde9/dbct_a_25709498_f0005_c.jpg)
Figure 6 UV–vis absorption spectra of the GNP–Her-conjugated Cy5 dye (A), Cy5 dye (B), and GNP (C) in aqueous solution.
![Figure 6 UV–vis absorption spectra of the GNP–Her-conjugated Cy5 dye (A), Cy5 dye (B), and GNP (C) in aqueous solution.](/cms/asset/29bc4c32-abfb-4233-bb4c-3b92d53912da/dbct_a_25709498_f0006_c.jpg)
Figure 7 Fluorescence microscopy.
Abbreviations: GNP, gold nanoparticle; GNP–Her, herceptin-conjugated GNP; FB, fibroblast cell line.
![Figure 7 Fluorescence microscopy.](/cms/asset/2709a95f-9286-4986-bdcf-edc5c82818a1/dbct_a_25709498_f0007_c.jpg)
Figure 8 MTT assay.
Abbreviations: GNP, gold nanoparticle; GNP–Her, herceptin-conjugated GNP; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
![Figure 8 MTT assay.](/cms/asset/9a290d25-1878-4934-99e2-8a513b941a47/dbct_a_25709498_f0008_c.jpg)
Figure 9 Amount of GNP (black bar) and GNP–Her (gray bar) taken up by SK-BR3 cells at different incubation times, as determined by ICP–MS.
![Figure 9 Amount of GNP (black bar) and GNP–Her (gray bar) taken up by SK-BR3 cells at different incubation times, as determined by ICP–MS.](/cms/asset/3416deb1-9157-4bcb-948a-b690c38bf819/dbct_a_25709498_f0009_c.jpg)
Figure 10 Fluorescence images of SK-BR3 cells.
Abbreviations: HER2, human epidermal growth factor receptor 2; DAPI, 4,6-diamidino-2-phenylindole dihydrochloride.
![Figure 10 Fluorescence images of SK-BR3 cells.](/cms/asset/1a3199f1-b24c-42b5-8cad-15303cd6687c/dbct_a_25709498_f0010_c.jpg)