The small leucine-rich proteoglycan, biglycan, is highly expressed in adipose tissue of Psammomys obesus and is associated with obesity and type 2 diabetes

Figures & data

Figure 1 Biglycan gene expression in a variety of P. obesus tissues.

Notes: RNA was extracted from a variety of tissues from T2D P. obesus and gene expression measured by RT-PCR. Biglycan expression is represented relative to hypothalamic expression.
Abbreviations: P. obesus, Psammomys obesus; IM, intramuscular; EDL, extensor digitorum longus; gastroc, gastrocnemius; T2D, type 2 diabetes; RT-PCR, real time polymerase chain reaction.

Figure 2A Analysis of biglycan gene expression in P. obesus visceral and subcutaneous adipose tissue.

Notes: RNA was extracted from visceral (mesenteric) and subcutaneous (subscapular) adipose tissue of NGT, IGT and T2D P. obesus and biglycan gene expression measured by RT-PCR. Data are mean ± SEM (n = 5–6 per group). Data is represented relative to expression in NGT subcutaneous adipose tissue. *P = 0.049 compared to subcutaneous IGT; ^P = 0.009, ^†P < 0.001 compared to subcutaneous NGT, respectively; ^#P = 0.002 compared to visceral NGT.
Abbreviations: P. obesus, Psammomys obesus; NGT, normal glucose tolerant; IGT, impaired glucose tolerant; T2D, type 2 diabetes; RT-PCR, real time polymerase chain reaction; SEM, standard error of mean.

Figure 2B Biglycan expression in fractionated P. obesus epididymal adipose tissue.

Notes: Epididymal adipose tissue from P. obesus was fractionated into adipocytes and stromal/vascular cells and gene expression measured by RT-PCR. Data are mean ± SEM (n = 5 per group). Overall, biglycan gene expression was significantly higher in the stromal/vascular compared to adipocyte fraction (*P < 0.023).
Abbreviations: P. obesus, Psammomys obesus; RT-PCR, real time polymerase chain reaction; SEM, standard error of mean.