Enhanced effect of fibroblast growth factor-2-containing dalteparin/protamine nanoparticles on hair growth

Figures & data

Figure 1 Diameter and zeta charge of FGF-2&D/P NPs stored at room temperature.

Notes: Measurements were performed weekly for 10 weeks. The diameter of FGF-2&D/P NPs was stable (~210–220 nm), and the zeta charge was also stable (~−25 mV to −28 mV).
Abbreviations: D/P, dalteparin/protamine; FGF-2, fibroblast growth factor-2; NPs, nanoparticles; W, week.

Figure 2 The protective effect of D/P NPs on FGF-2 bioactivity.

Notes: FGF-2, D/P NPs, and FGF-2&D/P NPs were incubated at 4°C or 37°C for 2 weeks. The growth-stimulatory activity for HMVECs in vitro was stable for at least 2 weeks at 37°C compared to the growth-stimulatory activity for those without D/P NPs. Positive control implies fresh FGF-2. Negative control indicates the absence of FGF-2 and D/P NPs.
Abbreviations: D/P, dalteparin/protamine; FGF-2, fibroblast growth factor-2; HMVECs, human microvascular endothelial cells; NPs, nanoparticles; OD, optical density.

Figure 3 FGF-2 antibody staining of the F344 rat dorsal skin with applied saline (control), standalone FGF-2, or FGF-2&D/P NPs.

Notes: The FGF-2&D/P NPs-applied group was stained more strongly than FGF-2 and saline-applied groups.
Abbreviations: D/P, dalteparin/protamine; FGF-2, fibroblast growth factor-2; NPs, nanoparticles.

Figure 4 Results of the pretreatment and post 6 months of treatment.

Notes: (A) Representative photographs of improved cases. (B) Diameter of hair. (C) Percentage of terminal hair. The application of FGF-2&D/P NPs for 6 months increased the mean diameter of hairs and the percentage of terminal hair (n=12). The data represent the mean ± SD. *P<0.05, **P<0.01.
Abbreviations: D/P, dalteparin/protamine; FGF-2, fibroblast growth factor-2; NPs, nanoparticles.