More Than Skin Deep – the Effects of Ultraviolet Radiation on Cathepsin K and Progerin Expression in Cultured Dermal Fibroblasts

Figures & data

Table 1 Expression of Cathepsin K and Progerin Protein/mRNA After Single High Dose Irradiation

	UVA				UVBbwb				UVBnb
	Cathepsin K		Progerin		Cathepsin K		Progerin		Cathepsin K		Progerin
	Protein	mRNA	Protein	mRNA	Protein	mRNA	Protein	mRNA	Protein	mRNA	Protein	mRNA
0 h	76.92±5.34	−5.4	22.12±2.69	−4.2	27.25±3.02	−4.0	56.25±8.41	−1.8	91.52±11.23	−2.6	66.45±2.16	−2.9
24 h	78.86±9.10	−8.1	22.67±0.82	−3.4	19.47±1.12	0.4	57.57±5.96	−3.5	87.92±6.07	−7.8	52.92±4.21	−5.3
48 h	87.91±6.68	−1.8	71.94±7.37	−2.7	55.77±1.00	−3.8	56.31±5.13	−1.9	63.41±0.26	−0.9	54.42±1.70	−2.4
p-value	0.2177	0.0087	0.0001	0.2435	0.0009	0.0625	0.9629	0.3135	0.0067	0.0659	0.0023	0.1372

Table 2 Expression of Cathepsin K and Progerin Protein/mRNA After Repeated Low Dose Irradiation vs High Dose Irradiation

Irradiation			Single High Dose	Repeated Low Dose	p-value
UVA	Cathepsin	Protein	87.91±6.68	69.91±4.29	0.0171
		mRNA	−1.8	−1.4	0.8585
	Progerin	Protein	71.94±7.37	42.51±4.35	0.0033
		mRNA	−2.7	0.3	0.0116
UVBbwb	Cathepsin	Protein	55.77±1.00	61.18±6.23	0.2115
		mRNA	−3.8	−2.1	0.5149
	Progerin	Protein	56.31±5.13	22.34±2.15	0.0004
		mRNA	−1.9	−1.6	0.8165
UVBnb	Cathepsin	Protein	63.41±0.26	46.63±2.05	0.0001
		mRNA	−0.9	−4.4	0.0129
	Progerin	Protein	54.42±5.70	20.93±2.96	<0.0001
		mRNA	−2.4	−0.4	0.0006

Figure 1 Alterations in progerin (A) and cathepsin K (B) mRNA relative expression (-ΔCt) after exposure to different wavelengths of UVR. Asterisks above columns indicate level of statistical significance (* p<0.05, **p<0.001). Data presented as means (horizontal black line within box) with standard deviation (boxes).

Figure 2 Alterations in progerin (A) and cathepsin K (B) protein expression after exposure to different wavelengths of UVR. Asterisks above columns indicate level of statistical significance (* p<0.05, **p<0.001, ***p<0.0001). Data presented as means (boxes) with standard deviation (whiskers). Corresponding Western blot bands are shown below each graph.