Signaling Molecules of Human Skin Cells as the Targets for Injection Cosmetology

Figures & data

Figure 1 Expression of melatonin receptor 1A in keratinocytes enriched with Merkel cell culture at passage 1 and 3; DAPI stained nuclei; expression of cytokeratin-20 – green fluorescence; expression of the protein under study – red fluorescence; magnification x40.

Table 1 The Influence of HA Fillers (HA-Y and HA-S) on the Area of Signaling Molecules Expression in Mixed Culture of Keratinocytes and Fibroblasts

Marker	Group	Area of Expression (%)
		Passage 3 (Mean±SD)	Passage 6 (Mean±SD)
AP-1	Control	15.54±1.18	21.86±1.82
	HA-Y	16.02±1.45	16.94±1.32
	HA-S	15.86±1.12	16.52±1.04
Klotho	Control	1.12±0.16	1.36±0.09
	HA-Y	1.34±0.08	5.05±0.37
	HA-S	1.47±0.12	4.98±0.41
Clock	Control	11.41±1.13	7.25±0.83
	HA-Y	13.43±0.98	9.24±1.19
	HA-S	15.28±1.21	10.78±1.07
Elastin	Control	48.63±4.54	26.05±3.62
	HA-Y	57.32±3.91	35.8±3.7
	HA-S	55.39±4.65	39.51±3.58
MTH-1	Control	20.74±2.4	14.53±2.81
	HA-Y	21.28±2.72	19.87±2.69
	HA-S	22.06±2.92	19.43±2.66

Figure 2 Immunofluorescent analysis of Elastin marker expression in cell culture. Cell nuclei were counterstained with Hoechst. Magnification x40.

Figure 3 Expression levels of AP-1 and klotho proteins in the mixed culture of human fibroblasts and keratinocytes in the HA-S and HA-Y treated group and the control (Con) group tested by Western blot.