Figures & data
Table 1 Explant Distribution Designated to Study RFV3 for Barrier Repair Properties
Figure 1 Degree of PPAR-α transcription activation induced by test materials depicted by the Reporter Assay System. Data are presented as mean value ± SEM, n=13 for water, n= 3 for test samples; *p-value < 0.05, **p-value < 0.01, n.s. = not significant, † = cytotoxic conditions observed.
![Figure 1 Degree of PPAR-α transcription activation induced by test materials depicted by the Reporter Assay System. Data are presented as mean value ± SEM, n=13 for water, n= 3 for test samples; *p-value < 0.05, **p-value < 0.01, n.s. = not significant, † = cytotoxic conditions observed.](/cms/asset/ad47dc06-b5c1-4398-8ad7-71b3bdbc9d88/dcci_a_12161826_f0001_c.jpg)
Table 2 Comparison of Pterocarpus Marsupium Bark Extract (P90) to RFV3 at Equivalent Concentrations of Trans-Pterostilbene 10.3 µm on the Transcriptional Response of the PPAR-α-Controlled Luciferase Reporter Gene. Data are Presented as Mean Values, n = 5 for Water; n = 6 for Test Samples
Table 3 Description of 36 of 96 Significantly and Near-Significantly Modulated Genes. Fold Change vs Control for Each Gene on the PCR Panel. Modulation of Gene Expression by RFV3 vs Glycerin Negative Control Expressed as Fold Change (FC; Up- or Down-Regulation)
Figure 2 Graphic representation of connectivity in genes modulated by RFV3 (2%) in epidermal tissue substitutes vs glycerin control (in silico analysis with StringDB software).
![Figure 2 Graphic representation of connectivity in genes modulated by RFV3 (2%) in epidermal tissue substitutes vs glycerin control (in silico analysis with StringDB software).](/cms/asset/7c2f8593-26f7-4d18-9081-49430f43a595/dcci_a_12161826_f0002_c.jpg)
Table 4 Binding Affinity and Amino Acid Interacting Residues of GW590735, Endogenous Ligands (CLAs), Test Compounds and Complexes of Compounds. Bolded Amino Acid Interacting Residues Represent Common Binding Sites of GW590735, a Known Synthetic PPAR-α Agonist
Figure 3 Interacting amino acid residues and conformations within the LBD of PPAR-α. From left to right: (A) ximenynic acid + glyceryl linolenate at distance criterion of 2.5 Ȧ, (B) RFV3 at distance criterion of 2.5 Ȧ and (C) RFV3 at distance criterion of 6 Ȧ.
![Figure 3 Interacting amino acid residues and conformations within the LBD of PPAR-α. From left to right: (A) ximenynic acid + glyceryl linolenate at distance criterion of 2.5 Ȧ, (B) RFV3 at distance criterion of 2.5 Ȧ and (C) RFV3 at distance criterion of 6 Ȧ.](/cms/asset/bea470e9-bc93-4197-9840-e768d9ddbcce/dcci_a_12161826_f0003_c.jpg)
Figure 4 Microscopic evaluation of tissue and cell morphology of day 0 and day 1 explants. No alterations observed in the epidermis and dermis across all samples.
![Figure 4 Microscopic evaluation of tissue and cell morphology of day 0 and day 1 explants. No alterations observed in the epidermis and dermis across all samples.](/cms/asset/de2b73dc-190c-4505-a334-67e6a0379eaa/dcci_a_12161826_f0004_c.jpg)
Figure 5 Immunostaining of ceramides in the stratum corneum with day 1 explants. Starting top left samples: Untreated control, delipidated control, delipidated excipient treated, delipidated 0.5% RFV3 treated, delipidated 0.3% RFV3 treated, delipidated 5% RFV3 treated.
![Figure 5 Immunostaining of ceramides in the stratum corneum with day 1 explants. Starting top left samples: Untreated control, delipidated control, delipidated excipient treated, delipidated 0.5% RFV3 treated, delipidated 0.3% RFV3 treated, delipidated 5% RFV3 treated.](/cms/asset/c17f71ae-d57b-47fd-a486-1a3d4d63a05e/dcci_a_12161826_f0005_c.jpg)
Figure 6 Image analysis for % surface positive immunostaining of ceramides in the stratum corneum of day 1 explants. Error bars represent SD. Explants analyzed: 18 (6 batches, 3 explants per batch). Image analyses n=9 (3 images per explant). Treated samples vs U(C): § for p < 0.05 and §§ for p < 0.01. Treated vs D(C): †† for p < 0.01. Treated samples vs D(E) ** for p < 0.01.
![Figure 6 Image analysis for % surface positive immunostaining of ceramides in the stratum corneum of day 1 explants. Error bars represent SD. Explants analyzed: 18 (6 batches, 3 explants per batch). Image analyses n=9 (3 images per explant). Treated samples vs U(C): § for p < 0.05 and §§ for p < 0.01. Treated vs D(C): †† for p < 0.01. Treated samples vs D(E) ** for p < 0.01.](/cms/asset/edd7948d-f93f-40c5-b675-d52e4707a81c/dcci_a_12161826_f0006_c.jpg)
Figure 7 Immunostaining of filaggrin at the bottom of the stratum corneum with day 1 explants. Starting top left samples: Untreated control, delipidated control, delipidated excipient treated, delipidated 0.5% RFV3 treated, delipidated 0.3% RFV3 treated, delipidated 5% RFV3 treated.
![Figure 7 Immunostaining of filaggrin at the bottom of the stratum corneum with day 1 explants. Starting top left samples: Untreated control, delipidated control, delipidated excipient treated, delipidated 0.5% RFV3 treated, delipidated 0.3% RFV3 treated, delipidated 5% RFV3 treated.](/cms/asset/3d1ada3a-b9c9-4487-8a2e-cdd08028c3df/dcci_a_12161826_f0007_c.jpg)
Figure 8 Image analysis for % surface positive immunostaining of filaggrin at the bottom of the stratum corneum with day 1 explants. Error bars represent SD. Explants analyzed: 18 (6 batches, 3 explants per batch). Image analyses n=9 (3 images per explant). Treated samples vs U(C): § for p < 0.05. Treated vs D(C): †† for p < 0.01. Treated samples vs D(E) ** for p < 0.01.
![Figure 8 Image analysis for % surface positive immunostaining of filaggrin at the bottom of the stratum corneum with day 1 explants. Error bars represent SD. Explants analyzed: 18 (6 batches, 3 explants per batch). Image analyses n=9 (3 images per explant). Treated samples vs U(C): § for p < 0.05. Treated vs D(C): †† for p < 0.01. Treated samples vs D(E) ** for p < 0.01.](/cms/asset/49f04571-28ae-47d1-a389-f627748e5495/dcci_a_12161826_f0008_c.jpg)
Figure 9 Immunostaining of transglutaminase-1 in the epidermis granular layer with day 1 explants. Starting top left samples: Untreated control, delipidated control, delipidated excipient treated, delipidated 0.5% RFV3 treated, delipidated 0.3% RFV3 treated, delipidated 5% RFV3 treated.
![Figure 9 Immunostaining of transglutaminase-1 in the epidermis granular layer with day 1 explants. Starting top left samples: Untreated control, delipidated control, delipidated excipient treated, delipidated 0.5% RFV3 treated, delipidated 0.3% RFV3 treated, delipidated 5% RFV3 treated.](/cms/asset/dcff832f-55d8-4585-b5ec-e45dfe9654a1/dcci_a_12161826_f0009_c.jpg)
Figure 10 Image analysis for % surface positive immunostaining of transglutaminase-1 in the epidermis granular layer with day 1 explants. Error bars represent SD. Explants analyzed: 18 (6 batches, 3 explants per batch). Image analyses n=9 (3 images per explant). Treated samples vs U(C): §§ for p < 0.01. Treated vs D(C): † for p < 0.05 and †† for p < 0.01. Treated samples vs D(E) ** for p < 0.01.
![Figure 10 Image analysis for % surface positive immunostaining of transglutaminase-1 in the epidermis granular layer with day 1 explants. Error bars represent SD. Explants analyzed: 18 (6 batches, 3 explants per batch). Image analyses n=9 (3 images per explant). Treated samples vs U(C): §§ for p < 0.01. Treated vs D(C): † for p < 0.05 and †† for p < 0.01. Treated samples vs D(E) ** for p < 0.01.](/cms/asset/d3a831a6-d82a-4b02-b2e4-7e0832d78c9e/dcci_a_12161826_f0010_c.jpg)