Comparison of SOX-10, HMB-45, and Melan-A in Benign Melanocytic Lesions

Figures & data

Table 1 Clinical Data and Staining Results of Patients with a Diagnosis of Lentigo Senilis or Lentigo with Mild Junctional Proliferation

Case	Age	Sex	Site	Avg No. of Melanocytes Stained with SOX-10	Avg No. of Melanocytes Stained with HMB-45	Avg No. of Melanocytes Stained with Melan-A
1	49	F	L upper arm	27	14.5	20.5
2	66	M	L temple	72	20.5	40.5
3	69	F	L lateral superior chest	24.5	17	33.5
4	52	F	L leg	40.5	47.5	27.5
5	86	F	L medial lower leg	24.5	19.5	26.5
6	33	M	L lateral back	32.5	20.5	24.5
7	56	M	R chest	18.5	10.5	14.5
8	42	F	R superior upper back	19	9.5	17
9	67	F	L proximal medial calf	24.5	18	22.5
10	50	F	L distal pretibial region	33.5	30	47.5
11	50	F	L pretibial region	17.5	6	17
12	42	F	R medial fourth toe	29.5	11	27.5
13	61	F	R anterior upper leg	87.5	49.5	54
14	60	F	Anterior neck sternal notch	7.5	6	10.5
15	56	F	L cheek	15.5	24.5	23
16	59	M	L cheek	30	29.5	50.5
17	65	F	R lateral thoracic	28	13.5	36.5
18	51	F	L anterior proximal thigh	23	24	26
19	52	F	R medial superior chest	59	28.5	26
20	80	F	Upper sternum	11.5	13.5	7.5
21	64	F	L distal posterior upper arm	29	27	34
22	55	F	L chest	11.5	14	18
23	69	M	R distal pretibial region	8.5	10	18
24	69	M	R distal pretibial region	11	12	13.5
25	79	M	R posterior neck	46.5	8.5	34.5
26	70	M	Midline mid chest	16.5	11	22
27	80	M	R anterior shoulder	11	8.5	18
28	60	F	R clavicle	21	11	21.5
29	80	M	L chest near midline	11	5	13
30	65	F	R calf	24.5	21.5	27.5
31	64	F	L distal dorsal forearm	79	35.5	46.5
32	55	F	L superior lateral lower back	84	24.5	48

Figure 1 Mild atypical junctional proliferation. (A) H&E stain, (B) HMB-45 stain, (C) Melan-A stain, (D) SOX-10 stain. More intense staining noted for Melan-A and SOX-10 at the dermo-epidermal junction. Scale bar = 50 µm.

Figure 2 Lentigo with focal junctional melanocytic hyperplasia. (A) H&E stain, (B) HMB-45 stain, (C) Melan-A stain, (D) SOX-10 stain. Melan-A and SOX-10 showed increased staining at the dermo-epidermal junction. Scale bar = 50 µm.

Figure 3 In a logarithmic scale, SOX-10 stained 1.215 times more melanocytes than HMB-45 (p=0.0026) and Melan-A stained 1.214 times more melanocytes than HMB-45 (p=0.0011) with a 95% confidence interval.