Efficacy of chlorhexidine gluconate ointment (Oronine H^®) for experimentally-induced comedones

Figures & data

Figure 1 Study design. (A) Inhibitory effects of chlorhexidine gluconate ointment on comedone formation. Once-daily application of 50% oleic acid, followed by application of the test substance (chlorhexidine gluconate ointment or liquid paraffin), was carried out for 21 consecutive days (n = 3 in each group). (B) Therapeutic effect of the ointment on preformed comedones.

Note: After once-daily application of 50% oleic acid for seven days (days 1–7), the test substance was applied once a day for 14 days (days 8–21, n = 3 in each group).

Figure 2 Inhibitory effects of chlorhexidine gluconate ointment on comedone formation. (A) Dermatoscopic findings on days 0 and 21 for rabbit ear skin treated with 50% oleic acid and liquid paraffin (control group) or with 50% oleic acid and chlorhexidine gluconate ointment (treatment group). (B) Histological findings of hair pores treated with 50% oleic acid and liquid paraffin (control group), and with 50% oleic acid and chlorhexidine gluconate ointment (treatment group) on day 21. Bar = 200 μm. (C) Quantitative assessment of the inhibitory effects of treatment on comedone formation.

Notes: Comedone size was measured for 20 comedones per ear of each rabbit to compare the effects of chlorhexidine gluconate ointment and liquid paraffin (n = 120 total comedones). *P < 0.001, **P < 0.005. The experiments were performed twice with similar results.

Figure 3 Therapeutic effect on comedones.

Notes: Oleic acid 50% was applied alone once a day for seven days to induce comedones on the orifices of the external auditory canals of rabbit ears (days 1–7). Thereafter, chlorhexidine gluconate ointment was applied for 14 days on the same area until day 21 (treatment group). Liquid paraffin was applied for 14 days in the control group (n = 120 total comedones). *P < 0.005. The experiments were performed twice with similar results.