Effect of Yak Meat to the Daily Ration of Scalded Rats for Wound Healing

Figures & data

Table 1 Feed Composition and Nutrient Level

Group	Basic Feed		Yak Meat Feed	Yellow Beef Feed
Raw material composition: Soybean meal, fish meal, brewer’s yeast powder, vegetable oil, bran, corn, wheat, VA, VD, VE, VB1, VB2, VB6, pantothenic acid, calcium hydrogen phosphate, stone powder, iron, copper, manganese, zinc, etc.			40% yak meat 60% basic feed	40% yellow beef 60% basic feed
Water (%)		7.96	10.58	10.19
Ash (%)		8.55	8.43	8.45
Crude protein CP (%)		18.53	24.09	24.44
Ether extract EE (%)		2.30	6.60	4.61
Neutral detergent fiber (%)		52.35	51.82	58.70
Acidic detergent fiber (%)		37.21	35.62	53.78
Ca(%)		1.09	1.27	0.91
P(%)		0.97	0.82	0.74
Energy (MJ/kg)		9.69	11.23	10.62

Figure 1 Comparison of feed intake of rats.

Figure 2 Comparison of body weight of rats.

Table 2 Lee’s Index Added in Different Diets for Scald Rats

Group	n	Before Scald	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald	SEM	P
								Diet	Time	T×D
Yak meat feed group	6	301.62^abc	303.73^ab	291.72^bc	306.01^a	289.35^c	10.89	0.00	0.00	0.00
Yellow beef feed group	6	311.06^a	298.91^ab	309.46^a	298.46^ab	288.03^b	10.89	0.00	0.00	0.00
Basic feed group	6	307.25^a	306.23^a	309.94^a	303.7^a	278.05^b	10.89	0.00	0.00	0.00

Notes: Different lowercase letters on the superscript on the same line indicate significant differences.

Figure 3 Comparison of obesity index in rats.

Figure 4 (a) Wound condition of rats in each group on the first day after scald. (b) Wound status of rats in each group on the 7th day after scald. (c)Wound status of rats in each group on the 14th day after scald. (d) Wound condition of rats in each group on day 21 after scald. (e) Wound condition of rats in each group on the 30th day after scald.

Notes: (A) Yak meat feed group, (B) Yellow beef feed group, (C) Basic feed group, (D) Blank group.

Figure 5 (a) He staining status of rats in each group at 7 days after scald. (b) He staining of rats in each group 14 days after scald. (c) He staining of rats in each group at 30 days after scald. (d) He staining status of rats in each group 45 days after scald.

Notes: (A) Yak meat feed group, (B) Yellow beef feed group, (C) Basic feed group, (D) Blank group.

Figure 6 (a) Condition of rats in each group at 7th PCNA day after scald. (b). Conditions of rats in each group at the 14th PCNA day after scald. (c). Condition of rats in each group on day 30th PCNA after scald. (d) Condition of rats in each group on 45th PCNA after scald.

Notes: (A) Yak meat feed group, (B) Yellow beef feed group, (C) Basic feed group, (D) Blank group.

Table 3 PCNA Positive Cell Rate (%)

Group	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald	SEM	P
						Diet	Time	T×D
Yak meat feed group	0.29168	0.282491	0.22594	0.190727	0.81	0.873	0.172	0.964
Yellow beef feed group	0.277019	0.268421	0.221503	0.228147	0.81	0.873	0.172	0.964
Basic feed group	0.230932	0.28855	0.260042	0.239397	0.81	0.873	0.172	0.964
Blank group	0.251257				0.81	0.873	0.172	0.964

Figure 7 (a) Apoptosis of rats in each group on the 7th day after scald. (b) Apoptosis of rats in each group on the 14th day after scald. (c) Apoptosis of rats in each group on the 30th day after scald. (d) Apoptosis of rats in each group on the 45th day after scald.

Notes: (A) Yak meat feed group, (B) Yellow beef feed group, (C) Basic feed group, (D) Blank group.

Table 4 Positive Cell Ratio and Positive Area Ratio of Scald Rats in Different Groups

Time Point	Positive Cell Ratio (%)				Positive Area Ratio (%)
Group	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald
Yak meat feed group	2.54	0.76	0.47	0.69	0.29	0.08	0.05	0.07
Yellow beef feed group	8.40	3.65	0.99	0.49	2.04	0.58	0.12	0.05
Basic feed group	5.4	2.37	0.28	1.43	0.81	0.24	0.03	0.14
Blank group	0.55				0.08

Table 5 Comparison of Positive Cell Density, Average Optical Density and Positive Surface Density of Scald Rats in Different Feed Groups

Time Point	Positive Cell Density (mm²)				Average Optical Density				Positive Area Density
Group	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald	7th Days After Scald	14th Days After Scald	30th Days After Scald	45th Days After Scald
Yak meat feed group	301.62	241.14	51.01	84.84	0.34	0.47	0.46	0.39	53,381.95	1318.55	851.28	943.23
Yellow beef feed group	717.04	372.13	112.92	53.19	0.22	0.37	0.38	0.38	15,103.68	7266.77	1544.86	653.21
Basic feed group	525.58	241.14	29.49	115.58	0.27	0.42	0.55	0.23	7225.94	3352.53	616.50	1144.94
Blank group	57.99				0.69				1940.12