Benefits of the Dermocosmetic Mineral 89 Probiotic Fractions Adjunct to Topical Retinoids for Anti-Aging Benefits

Figures & data

Table 1 Characteristics of the Subjects Included in the Study

Number of Subjects	38 (Female)
Age (mean ± SEM)	53 ± 1 years old
Skin phototypes	Phototype I | 0 (0%)
	Phototype II | 4 (10.5%)
	Phototype III | 20 (52.6%)
	Phototype IV | 10 (26.3%)
	Phototype V | 3 (7.9%)
	Phototype VI | 1 (2.6%)
Sensitive skin	Yes | 38 (100%)
	No | 0 (0%)

Table 2 Descriptive Statistics, Evolution Across Time (D28-D0) and Treatment (Side) Comparison for Parameters Evaluated by the Investigator

	After 28 Days (Short-Term Effect). Comparison to Baseline (D0)
	Treated			Non-Treated			Treatment Comparison
	Mean ± SD	% Difference	p-value	Mean ± SD	% Difference	p-value	% Difference	p-value
Erythema	0.5 ± 0.7	−48.1	<0.001 (S)	0.4 ± 0.7	−38.4	0.002 (S)	16.7	0.003 (S)
Fine lines	0.6 ± 0.5	−15.2	<0.001 (S)	0.4 ± 0.4	−9.0	<0.001 (S)	6.7	<0.001 (S)
Skin tone	1.0 ± 0.6	−22.1	<0.001 (S)	0.7 ± 0.6	−15.8	<0.001 (S)	7.8	<0.001 (S)
Radiance	1.3 ± 0.5	−25.5	<0.001 (S)	1.1 ± 0.6	−21.3	<0.001 (S)	5.3	<0.001 (S)
Pores’ appearance	0.6 ± 0.6	−13.2	<0.001 (S)	0.5 ± 0.6	−10.9	<0.001 (S)	2.6	0.001 (S)

Table 3 Descriptive Statistics, Evolution Across Time (D28-D7) and Treatment (Side) Comparison for the Responses from Self-Assessment Questionnaire

	After 28 Days (Short-Term Effect). Comparison to D7
	Treated			Non-Treated			Treatment Comparison
	Mean ± SD	% Difference	p-value	Mean ± SD	% Difference	p-value	p-value
Tightness	0.3 ± 1.1	−74.8	0.092 (LS)	0.4 ± 1.1	−79.4	0.034 (S)	0.167 (NS)
Dryness	2.0 ± 3.1	−70.1	<0.001 (S)	1.2 ± 2.2	−46.5	0.002 (S)	0.107 (NS)
Burning	0.8 ± 3.1	−38.8	0.086 (LS)	0.6 ± 2.8	−34.1	0.196 (NS)	0.882 (NS)
Itching	0.6 ± 3.0	−35.8	0.351 (NS)	0.6 ± 2.4	−39.3	0.234 (NS)	0.781 (NS)
Stinging	0.8 ± 2.9	−47.0	0.140 (NS)	0.2 ± 2.3	−12.0	0.728 (NS)	0.039 (S)

Table 4 Descriptive Statistics, Evolution Across Time (D28-D0) and Treatment (Side) Comparison for Corneometer^® Readings

	After 28 Days (Short-Term Effect). Skin Hydration by Corneometer^® Readings
	Treated			Non-Treated			Treatment Comparison
	Mean ± SD	% Difference	p-value	Mean ± SD	% Difference	p-value	% Difference	p-value
Comparison to D7	3.81 ± 9.75	+5.60	0.021 (S)	0.27 ± 8.45	+0.41	0.845 (NS)	16.7	0.028 (S)
Comparison to baseline (D0)	7.38 ± 9.78	+11.46	<0.001 (S)	0.84 ± 9.12	+1.31	0.571 (NS)	6.7	<0.001 (S)

Table 5 Descriptive Statistics and Evolution Across Time (D84-D0) for Parameters Evaluated by the Investigator

	After 84 Days (Long-Term Effect). Comparison to Baseline (D0)
	Mean ± SD	% Difference	p-value
Erythema	0.7 ± 0.7	−70.0	<0.001 (S)
Dryness	0.1 ± 0.3	NA	0.109 (NS)
Fine lines	1.4 ± 0.5	−32.0	<0.001 (S)
Skin tone	1.8 ± 0.7	−42.0	<0.001 (S)
Radiance	2.5 ± 0.7	−47.9	<0.001 (S)
Pores’ appearance	1.5 ± 0.7	−32.6	<0.001 (S)

Table 6 Descriptive Statistics and Evolution Across Time for the Responses from Self-Assessment Questionnaire

	After 84 Days (Long-Term Effect). Comparison to D7
	Mean ± SD	% Difference	p-value
Tightness	0.4 ± 1.0	−99.4	0.011 (S)
Dryness	1.7 ± 3.7	−59.3	0.016 (S)
Burning	1.3 ± 2.6	−63.8	0.002 (S)
Itching	1.2 ± 2.9	−65.7	0.019 (S)
Stinging	1.4 ± 2.8	−80.3	0.003 (S)

Figure 1 Corneometer^® readings evaluated on D0, D7, D28 and D84. Error bars: 95% CI (confidence interval).

Abbreviation: AU, arbitrary unit.

Figure 2 DLQI (Dermatology Life Quality Index) evaluated on D0, D7, D28 and D84. Error bars: 95% CI (confidence interval). A decrease of 41.7% was reported at D28 compared to D0 and a decrease of 47.2% was reported between D84 and baseline (non-significant).

Figure 3 Skindex 16 questionnaire evaluated on D0, D7, D28 and D84. Comparison with baseline. Symptoms are denoted in purple, emotions are pictured in orange and function domain is represented in blue. Error bars: 95% CI (confidence interval); *represents p value ≤0.050, **represents p-value ≤0.010 and ***represents p-value ≤0.001.

Table 7 Descriptive Statistics, Evolution Across Time (D28-D0) and Treatment (Side) Comparison for the Inflammatory and Oxidative Markers; Pg/Mg Protein: Picogram per Milligram of Protein; UI/Mg Prot: United Inch per Milligram of Protein

	Treated			Non-Treated			Treatment Comparison
	Mean ± SD	% Difference	p-value	Mean ± SD	% Difference	p-value	% Difference	p-value
IL-1alpha (pg/mg prot)	6.63 ± 360.06	+0.96	0.381 (NS)	−23.31 ± 441.28	−3.19	0.552 (NS)	4.51	0.787 (NS)
IL-1Ra (pg/mg prot)	8207.38 ± 13,329.09	+41.21	0.003 (S)	8638.49± 12,489.22	+40.70	<0.001 (S)	1.51	0.796 (NS)
IL-8 (pg/mg prot)	1.61± 5.23	+35.37	0.109 (NS)	0.59 ± 5.15	+11.93	0.542 (NS)	19.83	0.121 (NS)
PGE2 (pg/mg prot)	−200.57± 365.69	−24.28	0.006 (S)	−267.06 ± 473.69	−31.73	0.003 (S)	11.89	0.302 (NS)
SOD (UI/mg prot)	2.37 ± 6.77	+23.52	0.070 (LS)	1.84± 7.66	+18.38	0.206 (NS)	4.43	0.535 (NS)
Catalase (UI/mg prot)	0.37± 11.24	+1.62	0.862 (NS)	−0.42± 11.32	−1.82	0.842 (NS)	3.56	0.447 (NS)

Table 8 Descriptive Statistics and Evolution Across Time (D84-D0) for the Inflammatory and Oxidative Markers; Pg/Mg Protein: Picogram per Milligram of Protein; UI/Mg Prot: United Inch per Milligram of Protein

Parameter	Time Point	Mean	SD	% Difference Change	p-value (Significance)
IL-1alpha (pg/mg prot)	D0	708.79	604.54
	D28	694.10	465.99
	D84	571.90	328.94
	D84 - D0	−136.89	412.76	−19.31	0.117 (NS)
	D84 - D28	−122.20	246.11	−17.61	0.012 (S)
IL-1Ra (pg/mg prot)	D0	20,570.89	13,137.18
	D28	28,125.63	15,757.98
	D84	22,896.59	14,074.70
	D84 - D0	2325.71	9830.51	11.31	0.213 (NS)
	D84 - D28	−5229.03	6511.83	−18.59	<0.001 (S)
IL-8 (pg/mg prot)	D0	4.75	5.21
	D28	6.16	7.18
	D84	3.80	4.72
	D84 - D0	−0.95	3.64	−19.97	0.171 (NS)
	D84 - D28	−2.36	3.26	−38.31	0.001 (S)
PGE2 (pg/mg prot)	D0	833.91	379.95
	D28	625.55	295.03
	D84	525.30	232.22
	D84 - D0	−308.61	381.23	−37.01	<0.001 (S)
	D84 - D28	−100.24	219.21	−16.03	0.020 (S)
SOD (UI/mg prot)	D0	10.04	7.42
	D28	12.43	7.77
	D84	10.34	5.24
	D84 - D0	0.30	6.58	3.02	0.806 (NS)
	D84 - D28	−2.09	5.66	−16.81	0.057 (LS)
Catalase (UI/mg prot)	D0	22.94	11.75
	D28	22.96	10.89
	D84	20.91	9.13
	D84 - D0	−2.03	11.69	−8.86	0.357 (NS)
	D84 - D28	−2.05	7.96	−8.95	0.175 (NS)