The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

Figures & data

Table 1 The Positive Rate and Intensity of CD2AP Expression in 25 Cases of Chronic Eczema and 18 Cases of Normal Skin Around Eczema

Groups	n	Epidermic		Epidermic
		Positive Rate [n(%)]	Expression	Positive Rate [n(%)]	Expression
Skin lesion	25	25 (100%)	2.8 (2.0, 3.7)	25 (100%)	4.3 (3.5, 5.1)
Perilesional normal skin	18	13 (72.2%)	0.9 (0.4, 1.3)	6(33.3%)	0.5 (0.0, 1.1)
P		<0.05	<0.05	<0.05	<0.05

Figure 1 Expressions of CD2AP in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) More CD2AP+ monocyte-like cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More CD2AP+ monocyte-like cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of CD2AP+ monocyte-like cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

Table 2 The Positive Rate and Intensity of CD123 Expression in 22 Cases of Eczema and 18 Cases of Normal Skin Around Eczema

Groups	n	Epidermic		Epidermic
		Positive Rate [n(%)]	Expression	Positive Rate [n(%)]	Expression
Skin lesion	22	17 (77.3%)	0.8 (0.5, 1.2)	22 (100%)	2.5 (1.7, 3.4)
Perilesional normal skin	18	7 (29.2%)	0.3 (0.0, 0.5)	9 (50%)	0.8 (0.2, 1.4)
P		<0.05	<0.05	<0.05	<0.05

Figure 2 Expressions of CD123 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) A few CD123+ monocyte-like cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More CD123+ monocyte-like cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of CD123+ monocyte-like cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

Table 3 The Positive Rate and Intensity of TLR7 Expression in 17 Cases of Chronic Eczema and 13 Cases of Normal Skin Around Eczema

Groups	n	Epidermic		Epidermic
		Positive Rate [n(%)]	Expression	Positive Rate [n(%)]	Expression
Skin lesion	17	15 (88.2%)	1.4 (0.8, 2.1)	15 (88.2%)	1.4 (0.9, 1.9)
Perilesional normal skin	13	7 (53.8%)	0.7 (0.2, 1.1)	4 (30.8%)	0.2 (0.0, 0.4)
P		<0.05	>0.05	<0.05	<0.05

Figure 3 Expressions of TLR7 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) A few TLR7+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) A few TLR7+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Few TLR7+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

Table 4 The Positive Rate and Intensity of TLR9 Expression in 16 Cases of Chronic Eczema and 14 Cases of Normal Skin Around Eczema

Groups	n	Epidermic		Epidermic
		Positive Rate [n(%)]	Expression	Positive Rate [n(%)]	Expression
Skin lesion	16	16 (100%)	1.7 (1.3, 2.1)	15 (93.8%)	2.5 (1.5, 3.5)
Perilesional normal skin	14	12 (85.7%)	1.1 (0.7, 1.4)	8 (57.1%)	0.5 (0.2, 0.9)
P		>0.05	>0.05	<0.05	<0.05

Figure 4 Expressions of TLR9 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

Table 5 The Positive Rate and Intensity of IRAK1 Expression in 23 Cases of Chronic Eczema and 20 Cases of Normal Skin Around Eczema

Groups	n	Epidermic		Epidermic
		Positive Rate [n(%)]	Expression	Positive Rate [n(%)]	Expression
Skin lesion	23	22 (95.7%)	1.8 (1.3, 2.3)	22 (95.7%)	3.7 (2.7, 4.6)
Perilesional normal skin	20	10 (50%)	0.8 (0.5, 1.2)	9 (45)	0.4 (0.2, 0.6)
P		<0.05	<0.05	<0.05	<0.05

Figure 5 Expressions of IRAK1 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) More IRAK1+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More IRAK1+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of IRAK1+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

Table 6 The Positive Rate and Intensity of IRF7 Expression in 22 Cases of Chronic Eczema and 16 Cases of Normal Skin Around Eczema

Groups	n	Epidermic		Epidermic
		Positive Rate [n(%)]	Expression	Positive Rate [n(%)]	Expression
Skin lesion	21	20 (95.2%)	1. 0(0.7, 1.3)	19 (90.5%)	3.0 (2.1, 4.0)
Perilesional normal skin	16	7 (43.8%)	0.3 (0.1, 0.5)	5 (31.3%)	0.4 (0.0, 0.8)
P		<0.05	<0.05	<0.05	<0.05

Figure 6 Expressions of IRF7 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion (c) Perilesional normal skin. (a) More IRF7+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More IRF7+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of IRF7+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.