Ex vivo Evaluation of a Liposome-Mediated Antioxidant Delivery System on Markers of Skin Photoaging and Skin Penetration

Figures & data

Table 1 Composition of Liposomes

Component	%p/v	Phase
Phospatidylcholine	8.697	A
Cutin	0.850	A
Tetrahexyldecyl ascorbate	0.120	A
Haematococcus pluvialis extract	2.450	A
Glycerol	4.000	B
Ectoin	1.240	B
Panthenol	0.120	B

Figure 1 Emission spectra of a SOL Solar Simulator.

Figure 2 Graphical scheme of treatment regimen.

Figure 3 Tissue damage (LDH leakage) of non-irradiated hOSEC (Healthy), irradiated (UV Control) or irradiated and treated with either the free antioxidant complex or the encapsulated antioxidant complex.

Figure 4 Metabolic activity (resazurin reduction) of non-irradiated hOSEC (Healthy), irradiated (UV Control) or irradiated and treated with either the free antioxidant or encapsulated antioxidant complex.

Figure 5 IL-6 secretion of non-irradiated hOSEC (Healthy), irradiated (UV Control) or irradiated and treated with either the free antioxidant complex or the encapsulated antioxidant complex.

Figure 6 IL-8 secretion of non-irradiated hOSEC (Healthy), irradiated (UV Control) or irradiated and treated with either the free antioxidant complex or encapsulated antioxidant complex. Astericks *Indicate a statistically significant difference (p<0.05) compared to the Healthy group, hashes ^#Compared to the UV Control group, and dollar sign ^$Compared to the free antioxidant complex group.

Figure 7 Matrix Metalloproteinase 9 (MMP-9) secretion of non-irradiated hOSEC (Healthy), irradiated (UV Control) or irradiated and treated with either the free antioxidant complex or encapsulated antioxidant complex. Astericks *Indicate a statistically significant difference (p<0.05) compared to the Healthy group, hashes ^#Compared to the UV Control group, and dollar sign ^$Compared to the free antioxidant complex group.

Figure 8 (A) Cross section of the skin sample. (B) Confocal image of encapsulated antioxidant complex liposomes (red) after penetration in a skin sample (grey) and (C) Confocal image of encapsulated antioxidant complex liposomes (red) after penetration in a skin sample (grey) stained with DAPI (blue).

Abbreviations: SC, stratum corneum; EP, epidermis; D, dermis.

Data Sharing Statement

The data is not publicly available.