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Original Research

In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

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Pages 389-396 | Published online: 16 Jul 2015

Figures & data

Table 1 In vitro antioxidant activity of fruits extract

Figure 1 Dose-dependent inhibition of collagenase by combined fruits extract. Fluorescence was measured at excitation maxima of 495 nm and emission maxima of 515 nm. The data are representative of three different experiments with similar results. *P<0.05 when compared with placebo.

Figure 1 Dose-dependent inhibition of collagenase by combined fruits extract. Fluorescence was measured at excitation maxima of 495 nm and emission maxima of 515 nm. The data are representative of three different experiments with similar results. *P<0.05 when compared with placebo.

Figure 2 Photograph showing the images of wrinkles used for assessment of wrinkle area, depth, and length in the crow’s feet region of subject’s eyes treated with 2% topically formulated fruits extract and placebo treated for 56 days. Clinical evaluations and measurements were performed on D0 (before treatment), D28, and D56.

Abbreviation: D, day.
Figure 2 Photograph showing the images of wrinkles used for assessment of wrinkle area, depth, and length in the crow’s feet region of subject’s eyes treated with 2% topically formulated fruits extract and placebo treated for 56 days. Clinical evaluations and measurements were performed on D0 (before treatment), D28, and D56.

Figure 3 Differences in the wrinkle depth after the treatment of 2% topical formulated fruit extracts containing cream or the placebo randomly on crow’s feet region of eyes of 21 healthy subjects. Data are expressed as mean ± SE. *P<0.05 when compared with placebo.

Figure 3 Differences in the wrinkle depth after the treatment of 2% topical formulated fruit extracts containing cream or the placebo randomly on crow’s feet region of eyes of 21 healthy subjects. Data are expressed as mean ± SE. *P<0.05 when compared with placebo.

Figure 4 Differences in the wrinkle length after the treatment of 2% topical formulated fruit extracts containing cream or the placebo randomly on crow’s feet region of eyes of 21 healthy subjects. Data are expressed as mean ± SE. *P<0.05 when compared with placebo.

Figure 4 Differences in the wrinkle length after the treatment of 2% topical formulated fruit extracts containing cream or the placebo randomly on crow’s feet region of eyes of 21 healthy subjects. Data are expressed as mean ± SE. *P<0.05 when compared with placebo.

Figure 5 Differences in the wrinkle area after the treatment of 2% topical formulated fruits extract containing cream or the placebo randomly on crow’s feet region of eyes of 21 healthy subjects. Data are expressed as mean ± SE. **P<0.01 when compared with placebo.

Figure 5 Differences in the wrinkle area after the treatment of 2% topical formulated fruits extract containing cream or the placebo randomly on crow’s feet region of eyes of 21 healthy subjects. Data are expressed as mean ± SE. **P<0.01 when compared with placebo.

Figure 6 Differences in the visual score after the treatment of 2% topically formulated fruits extract containing cream or the placebo randomly on the crow’s feet region of eyes of healthy subjects. Data are expressed as mean ± SE. *P<0.05 when compared with placebo.

Figure 6 Differences in the visual score after the treatment of 2% topically formulated fruits extract containing cream or the placebo randomly on the crow’s feet region of eyes of healthy subjects. Data are expressed as mean ± SE. *P<0.05 when compared with placebo.