Cosmeceutical product consisting of biomimetic peptides: antiaging effects in vivo and in vitro

Figures & data

Figure 1 A histological examination of the skin biopsy specimens, stained with H&E (magnification ×100).

Notes: (A) Control group. (B) Administration of hyaluronic acid. (C) Administration of AQ preparation. Scale bars 200 μm.
Abbreviations: H&E, hematoxylin and eosin; AQ, aquashine.

Figure 2 Volumetric reconstruction of marker expression of procollagen (CLSM).

Notes: (A) Control sample. (B) AQ administration.
Abbreviations: CLSM, confocal laser scanning microscopy; AQ, aquashine.

Table 1 The influence of biomimetic peptides (AQ and AQ BR) on the area of signaling molecules expression in cell culture of fibroblasts

Marker peptide	Group	Area of expression (%)
		Passage 7 (mean ± SD)	Passage 15 (mean ± SD)
Ki-67	Control	20.85±4	15.63±3
	AQ	38.47±5*	31.25±5*
	AQ BR	47.58±5*	33.30±5*
SIRT6	Control	9.65±0,5	2.05±0.4
	AQ	11.36±1*	9.29±1*
	AQ BR	12.13±2*	10.08±1*
Type I procollagen	Control	17.58±2	3.37±1
	AQ	22.57±2*	12.07±1*
	AQ BR	24.82±2*	10.63±2*
AP-1	Control	5.12±0.5	7.12±0.5
	AQ	2.34±0.5*	4.98±1*
	AQ BR	2.08±0.1*	6.18±1

Note:

* Comparison versus control, P<0.05.

Abbreviations: AQ, aquashine, SD, standard deviation; BR, brightening.

Figure 3 Immunohistochemical EnVision staining of fibroblast cell culture.

Notes: Immunoreactivity for Ki-67 was observed in mature cell culture (passage 7) in the control group (A), in group with addition of biomimetic peptides from AQ (B), and in group with addition of biomimetic peptides from AQ BR (C). Immunopositive nucleus was revealed in a senescent cell culture (passage 15) in the control group (D), in the AQ group (E), and in the AQ BR group (F). Scale bars: 100 μm. Magnification =200×.
Abbreviations: AQ, aquashine; BR, brightening.