Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

Figures & data

Table 1 Mean and Standard Deviation (SD) of APAF-1, Caspase-3, and Caspase-9 Expressions from HUCMSCs in Control Groups and Calcium Hydroxide Groups

Groups and Exposure Days	Expression of APAF-1Mean±SD	Expression of Caspase-3 Mean±SD	Expression of Caspase-9 Mean±SD
Control Day 1	1.5227±0.40756	1.3200±0.29311	1.3200±0.27310
Control Day 3	14.8600±0.57280	3.5400±0.58522	11.6800±1.27915
Control Day 7	15.3100±0.61397	2.0600±0.21517	28.0200±1.99328
Calcium hydroxide Day 1	3.8247±0.96960	1.6100±0.33143	1.5567±0.33949
Calcium hydroxide Day 3	18.8913±3.91792	31.8260±9.04446	29.7520±6.08016
Calcium hydroxide Day 7	24.8733±5.34421	31.2267±5.14817	30.9620±4.22264

Table 2 Significance (P-value) Among Exposure Day in the Control Groups and Calcium Hydroxide Groups on APAF-1 Expression

Groups and Exposure Day on APAF-1 Expression	Control Day 1	Control Day 3	Control Day 7	Calcium Hydroxide Day 1	Calcium Hydroxide Day 3	Calcium Hydroxide Day 7
Control Day 1	–	0.000*	0.000*	0.000*	–	–
Control Day 3	0.000*	–	0.027*	–	0.001*	–
Control Day 7	0.000*	0.027*	–	–	–	0.000*
Calcium hydroxide Day 1	0.000*	–	–	–	0.000*	0.000*
Calcium hydroxide Day 3	–	0.001*	–	0.000*	–	0.000*
Calcium hydroxide Day 7	–	–	0.000*	0.000*	0.000*	–

Note: *Significant (P<0.05).

Figure 1 HUCMSCs culture seen under light microscope (L) and fluorescence microscope (F) showing the expressions of APAF-1, Caspase-3, and Caspase-9 observed on day 1, day 3, and day 7 between control groups and calcium hydroxide (CH) groups.

Table 3 Significance (P-value) Among Exposure Day in the Control Groups and Calcium Hydroxide Groups on Caspase-3 Expression

Groups and Exposure Day on Caspase-3 Expression	Control Day 1	Control Day 3	Control Day 7	Calcium Hydroxide Day 1	Calcium Hydroxide Day 3	Calcium Hydroxide Day 7
Control Day 1	–	0.000*	0.000*	0.017*	–	–
Control Day 3	0.000*	–	0.000*	–	0.000*	–
Control Day 7	0.000*	0.000*	–	–	–	0.000*
Calcium hydroxide Day 1	0.017*	–	–	–	0.000*	0.000*
Calcium hydroxide Day 3	–	0.000*	–	0.000*	–	0.786
Calcium hydroxide Day 7	–	–	0.000*	0.000*	0.786	–

Note: *Significant (P<0.05).

Table 4 Significance (P-value) Among Exposure Day in the Control Groups and Calcium Hydroxide Groups on Caspase-9 Expression

Groups and Exposure Day on Caspase-9 Expression	Control Day 1	Control Day 3	Control Day 7	Calcium Hydroxide Day 1	Calcium Hydroxide Day 3	Calcium Hydroxide Day 7
Control Day 1	–	0.000*	0.000*	0.045*	–	–
Control Day 3	0.000*	–	0.000*	–	0.000*	–
Control Day 7	0.000*	0.000*	–	–	–	0.024*
Calcium hydroxide Day 1	0.045*	–	–	–	0.000*	0.000*
Calcium hydroxide Day 3	–	0.000*	–	0.000*	–	0.443
Calcium hydroxide Day 7	–	–	0.024*	0.000*	0.443	–

Note: *Significant (P<0.05).