Clinicopathological features and Borrmann classification associated with HER2-positive in primary gastric cancer

Figures & data

Figure 1 Human epidermal growth factor receptor 2 (HER2) expression by IHC (magnification ×200).

Figure 2 Human epidermal growth factor receptor 2 (HER2) status by FISH (magnification ×200).

Table 1 Clinical and pathological features of the study cases

		Total	Surgical samples	Endoscopic biopsy samples
Enrolled patients, n		461	99	362
Gender	Male	310	65	245
	Female	151	34	117
Age	≤60	117	33	84
	>60	344	66	278
Primary tumor location	Gastroesophageal junction	45	12	33
	Upper third	70	15	55
	Middle third	230	42	188
	Lower third	116	30	86
Tumor size, cm	<5	286	51	235
	≥5	175	48	127
Histological type	Intestinal type	175	47	128
	Diffuse type	171	41	130
	Mixed type	115	11	104
Histological grade	Well-differentiated	241	59	182
	Poorly differentiated	220	40	180
Borrmann classification	I	29	9	20
	II	128	26	102
	III	197	44	153
	IV	96	19	87
Lymph node metastasis	Present	324	76	248
	Absent	137	23	114
TNM stages	I+II	152	31	121
	III+IV	309	68	241

Table 2 HER2 status assessed by IHC and FISH in 461 primary gastric carcinomas (bioptic and surgical specimens)

	Tot	IHC 0	IHC 1+	IHC 2+		IHC 3+	HER2 negative (%)	HER2 positive (%)
				FISH-	FISH+
Biopsy specimens	362	220	47	41	20	34	308 (85%)	54 (15%)
Surgical specimens	99	50	12	17	8	12	79 (80%)	20 (20%)
Tot	461	270	59	66	37	46	387 (84%c)	74 (16%)

Table 3 HER2 positivity according to clinicopathological factors of patients with gastric cancer

Factor		HER2-positive (n=74)	HER2-negative (n=387)	p-Value
		74	387
Gender	Male	60	250	0.006
	Female	14	137
Age	≤60	23	94	0.219
	>60	51	293
Primary tumor location	Gastroesophageal junction	11	34	0.372
	Upper third	10	60
	Middle third	33	197
	Lower third	20	96
Tumor size, cm	<5	43	243	0.447
	≥5	31	144
Histological type	Intestinal type	37	138	0.062
	Diffuse type	21	150
	Mixed type	16	99
Histological grade	Well-differentiated	49	192	0.009
	Poorly differentiated	25	195
Borrmann classification	I	2	27	0.001
	II	9	121
	III	44	153
	IV	17	88
Lymph node metastasis	Present	56	268	0.268
	Absent	18	119
TNM stages	I+II	31	121	0.075
	III+IV	43	266

Figure 3 Association of HER2 status with different tumor subtypes in surgical resection samples.

Table 4 Relationship pf Borrmann classification to HER2 IHC status in surgical (P=0.171)

HER2 IHC Status	Borrmann classification assessment				Total
	1	2	3	4
0	6	9	26	8	61
1	2	6	3	2	12
2	1	10	7	6	25
3	0	3	8	2	12
Total	9	28	44	18	99

Figure 4 Association of HER2 status with different tumor subtypes in endoscopic biopsy samples.

Table 5 Relationship pf Borrmann classification to HER2 IHC status in biopsy (p=0.001)

HER2 IHC Status	Borrmann classification assessment				Total
	1	2	3	4
0	11	63	78	68	220
1	2	10	30	5	47
2	5	21	31	4	61
3	2	8	14	10	34
Total	20	102	153	87	362