The potential role of testosterone in hypertension and target organ damage in hypertensive postmenopausal women

Figures & data

Table 1 Data sharing statements that fulfill these ICMJE requirements

Whether the authors intend to share individual deidentified participant data?	Yes.
What data in particular will be shared?	Individual participant data that underlie the results reported in this article, after deidentification (text and tables).
What other documents will be available?	Study Protocol and Statistical Analysis Plan.
When will data be available (start and end dates)?	Beginning 9 months and ending 36 months following article publication.
By what mechanism will data be made available?	Proposals may be submitted up to 36 months following article publication. After 36 months the data will be available in our University’s data warehouse but without investigator support other than deposited metadata.

Table 2 Comparisons of clinical characteristics and metabolic indexes between two groups (N=322)

	Male (n=161)	Female (n=161)	P
Age (years)	55.95±5.89	55.91±5.89	0.947
BMI (kg/m^2)	25.75±2.36	25.20±2.78	0.052
WBC (×10^9/L)	6.37±1.52	6.05±1.80	0.090
RBC (×10^12/L)	4.69±0.42	4.61±0.50	0.109
HGB (g/L)	138.82±14.01	136.22±14.57	0.104
PLT(×10^9/L)	190.51±75.11	188.30±56.58	0.766
K^+ (mmol/L)	3.85±0.46	3.77±0.38	0.092
Na^+ (mmol/L)	141.44±16.40	141.05±3.02	0.770
Cr (μmmol/L)	78.77±11.04	68.14±13.92	0
BUN (mmol/L)	6.22±2.12	5.90±1.36	0.111
UA (μmmol/L)	380.78±93.25	365.63±53.06	0.074
TC (mmol/L)	4.11±1.03	4.32±1.02	0.068
TG (mmol/L)	1.96±1.17	1.79±1.20	0.204
HDL (mmol/L)	1.23±0.33	1.29±0.34	0.133
LDL (mmol/L)	2.21±0.79	2.36±0.82	0.082
GLU (mmol/L)	6.87±0.85	6.93±0.63	0.259
ALT (U/L)	37.02±27.99	31.44±30.52	0.088
AST (U/L)	30.14±22.82	27.16±17.96	0.195
SBP (mmHg)	142.24±19.27	138.47±17.97	0.070
DBP (mmHg)	85.65±11.98	80.68±11.62	0

Note: The data are shown as mean ± SD.

Abbreviations: BMI, body mass index; WBC, white blood cell; RBC, red blood cell; HGB, hemoglobin; PLT, platelet; Cr, creatinine; BUN, blood urea nitrogen; UA, uric acid; TC, total cholesterol; TG, triglyceride; HDL, high-density lipoprotein; LDL, low-density lipoprotein; GLU, glucose; ALT, alanine transaminase; AST, aspartate aminotransferase; SBP, systolic blood pressure; DBP, diastolic blood pressure; SD, standard deviation.

Table 3 The characteristics of antihypertensive drugs for all hypertensive patients in this study (N=322)

	Male (n=161)	Female (n=161)	P
ACEI	54 (33.5%)	41 (25.5%)	0.112
ARB	39 (24.2%)	52 (32.3%)	0.108
CCB	55 (34.2%)	48 (30.0%)	0.403
Diuretic	41 (25.5%)	32 (20.0%)	0.231
β-blocker	37 (23.0%)	29 (18.0%)	0.269
ACEI±CCB	19 (11.8%)	12 (7.5%)	0.186
ARB±CCB	11 (6.8%)	6 (3.7%)	0.213
ACEI± Diuretic	18 (11.2%)	12 (7.5%)	0.250
ARB± Diuretic	8 (5.0%)	14 (8.7%)	0.185

Note: The data are shown as n (%).

Abbreviations: ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin receptor blocker; CCB, calcium channel blocker.

Table 4 Comparisons of sex hormone levels between two groups (N=322)

	Male (n=161)	Female (n=161)	P
PRL (ng/ml)	8.50±3.31	15.69±9.81	0
FSH (mIU/ml)	6.15±3.12	43.94±18.41	0
LH (mIU/ml)	4.85±2.51	28.46±11.97	0
E2 (pg/ml)	33.79±12.59	30.95±24.12	0.186
Testosterone (ng/dl)	404.22±137.43	36.22±15.09	0
Progesterone (ng/ml)	0.38±0.21	0.37±0.26	0.714

Note: The data are shown as mean ± SD.

Abbreviations: PRL, prolactin; FSH, follicle stimulating hormone; LH, luteinizing hormone; E₂, estradiol; SD, Standard deviation.

Table 5 Comparisons of ambulatory blood pressure monitoring characteristics between two groups (N=322)

	Male (n=161)	Female (n=161)	P
24h-mean-SBP (mmHg)	134.76±16.58	128.09±14.23	0
24h-mean-DBP (mmHg)	80.53±10.38	75.63±7.79	0
daytime-mean-SBP (mmHg)	138.19±16.93	130.97±14.67	0
daytime-mean-DBP (mmHg)	83.12±10.63	77.89±7.97	0
nighttime-mean-SBP (mmHg)	126.35±15.64	123.77±15.72	0.140
nighttime-mean-DBP (mmHg)	75.93±10.77	72.33±9.52	0.002
24 h load of SBP (%)	51.84±26.86	42.67±26.92	0.002
24 h load of DBP (%)	49.24±25.53	36.70±22.35	0
daytime load of SBP (%)	49.99±27.54	41.06±27.06	0.004
daytime load of DBP (%)	45.03±26.85	29.85±20.90	0
nighttime load of -SBP (%)	55.35±28.51	50.43±30.31	0.135
nighttime load of DBP (%)	57.69±27.26	50.44±29.70	0.023
24 h variation of SBP (%)	13.41±4.29	14.67±4.67	0.012
24 h variation of DBP (%)	14.48±3.92	15.24±3.91	0.083
daytime variation of SBP (%)	13.05±4.29	14.20±4.77	0.024
daytime variation of DBP (%)	13.94±4.38	14.74±4.74	0.118
nighttime variation of SBP (%)	12.58±4.98	13.54±5.21	0.094
nighttime variation of DBP (%)	13.54±4.70	14.49±5.18	0.087

Note: The data are shown as mean ± SD.

Abbreviations: SBP, systolic blood pressure; DBP, diastolic blood pressure; SD, standard deviation.

Table 6 Comparisons of cardiac structure and function between two groups (N=322)

	Male (n=161)	Female (n=161)	P
LAD (mm)	34.37±5.58	31.50±4.61	0
LVEDd (mm)	48.17±5.14	46.61±4.81	0.005
IVST (mm)	9.01±1.49	8.58±1.52	0.011
PWT (mm)	8.93±1.33	8.90±1.28	0.821
LVEDV (ml)	111.13±28.56	95.46±25.13	0
LVESV (ml)	39.39±17.22	30.71±13.45	0
SV (ml)	70.93±14.92	64.95±14.82	0
EF (%)	64.89±9.14	66.04±6.33	0.191
FS (%)	36.18±7.22	37.50±5.39	0.063
CO (L/min)	5.00±1.25	4.74±1.11	0.053
LVM (g)	172.54±44.08	156.81±41.54	0.001
RWT	0.38±0.07	0.38±0.07	0.807
LVMI (g/m^2)	90.83±24.33	92.22±24.19	0.607
E (m/s)	0.73±0.13	0.75±0.16	0.131
A (m/s)	0.82±0.17	0.83±0.18	0.395
E/A	0.97±0.62	0.95±0.32	0.700
Em (m/s)	0.06±0.02	0.06±0.02	0.057
Am (m/s)	0.10±0.02	0.09±0.02	0.076
Em/Am	0.67±0.16	0.67±0.21	0.752
E/Em	12.05±3.68	13.06±3.53	0.012

Note: The data are shown as mean ± SD.

Abbreviations: LAD, left atrial diameter; LVEDd, left ventricular end-diastolic dimension; IVST, interventricular septal thickness; PWT, left ventricular posterior wall thickness; LVEDV, left ventricular end-diastolic volume; LVESV, left ventricular end-systolic volume; SV, stroke volume; EF, ejection fraction; FS, fractional shortening; LVM, left ventricular mass; RWT, relative wall thickness; LVMI, left ventricular mass index; E, early diastolic mitral peak flow velocity; A, late diastolic mitral peak flow velocity; Em, early diastolic mitral annular velocity; Am, late diastolic mitral annular velocity; SD, standard deviation.

Table 7 Comparisons of central hemodynamics and vascular function between two groups (N=322)

	Male (n=161)	Female (n=161)	P
CAP (mmHg)	12.35±5.28	13.14±4.97	0.172
AIx	30.07±8.32	32.49±9.09	0.013
AIx@HR75	28.00±8.32	31.81±7.52	0
Central-SBP (mmHg)	133.94±17.84	130.37±16.88	0.066
Central-DBP (mmHg)	85.36±12.19	80.53±10.70	0
cf-PWV (m/s)	8.03±2.82	9.68±2.23	0
HR (bpm)	71.02±8.39	72.69±9.73	0.100

Note: The data were shown as mean ± SD.

Abbreviations: CAP, central aortic pressure; HR, heart rate; AIx, augmentation index; AIx@HR75, AIx was normalized to a HR of 75 beats per minute; SBP, systolic blood pressure; DBP, diastolic blood pressure; cf-PWV, carotid-femoral pulse wave velocity; bpm, beats per minute; SD, standard deviation.

Table 8 The correlation ship between testosterone and TOD (N=322)

	γ	P
cf-PWV
testosterone	0.157	0.046
nighttime-mean-SBP
cf-PWV	0.210	0.008
E/Em
nighttime-mean-SBP	0.156	0.048

Abbreviations: cf-PWV, carotid-femoral pulse wave velocity; SBP, systolic blood pressure; E/Em, the ratio of early diastolic mitral peak flow velocity to early diastolic mitral annular velocity; TOD, target organ damage.