Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study

Figures & data

Table 1 Information of Dopamine and Beta-Actin Primers

Locus	Primer (Forward)	Primer (Reverse)	Product (bp)
β-actin	5ʹ-AGACGCAGGATGGCATGGG −3ʹ	5ʹ-GAGACCTTCAACACCCCAGCC −3ʹ	161
DRD1	5ʹ-CTTCCTCAACGTTTCGGAGCC-3ʹ	5ʹ-AGCTCTCCAAACGCCTTGCCTT-3ʹ	100
DRD2	5ʹ-TGTACAATACGCGCTACAGCTCCA-3ʹ	5ʹ-ATGCACTCGTTCTGGTCTGCGTTA-3ʹ	127
DRD3	5ʹ-TCTGTGCCATCAGCATAGACAGGT-3ʹ	5ʹ-TAAAGCCAAACAGAAGAGGGCAGG-3ʹ	156
DRD4	5ʹ-TCTTCGTCTACTCCGAGGTCCA-3ʹ	5ʹ-TGATGGCGCACAGGTTGAAGAT-3ʹ	125
DRD5	5ʹ-TCATCTATGCCTTCAACGCCGACT-3ʹ	5ʹ-AGCTGCGATTTCCTTGTGGAAGAC-3ʹ	120

Figure 1 Result of microarray data analysis and functional enrichment shows important genes which involve in dopaminergic synapse pathway related to OA.

Table 2 Description and Adjusted p-value, of Important Genes Which Involve in Dopaminergic Synapse Pathway Related to OA

Gene Symbol	Adj P Val	Description
ATF4	8.13E-11	Activating transcription factor 4
CREB1	6.87E-05	cAMP responsive element binding protein 1
GSK3B	2.17E-05	Glycogen synthase kinase 3 beta
GNG11	0.0429	Guanine nucleotide binding protein (G protein)
GNG8	0.010643	Guanine nucleotide binding protein (G protein),
GNAQ	0.031662	Guanine nucleotide-binding protein G
CLOCK	0.00988	Circadian locomoter output cycles protein kaput
PPP1R1B	0.009373	Protein phosphatase 1 regulatory subunit 1B;
GNAL	0.049965	Guanine nucleotide-binding protein G
PRKCG	0.048999	Protein kinase C gamma type
GNB1	0.022809	Uanine nucleotide-binding protein G

Figure 2 The network of the genes which linked directly with dopaminergic receptors genes in OA disease. The borders’ thicknesses of circles nodes indicate absolute LogFc.

Figure 3 The expression of the 6 most interested genes in OA disease (GSK3B, GNAQ, ATF4, GNG11, CREB1 and GNG8) which are in the network with significant relationship to Dopamine receptors.

Figure 4 Gene expression analysis of DRDs in OA patients in comparison to healthy controls. DRD1 and DRD5 receptor genes expression in the patient group were lower than those in the healthy controls; whiles DRD2, DRD3 and DRD4 receptor genes expression were higher.