Screening and Identification of Potential Hub Genes in Myocardial Infarction Through Bioinformatics Analysis

Figures & data

Table 1 Statistics and Further Screened of the Two Microarray Databases Derived from the GEO Database

Dataset ID	Infarction Time	Number
		Sham	MI	Total
GSE4648	1 day	2	2	4
GSE4648	2 day	2	2	4
GSE775	2 day	1	3	4
GSE775	7 day	1	3	4

Table 2 Reclassifying Two Gene Expression Profiles Based on Infarct Time

Infarction Time	Number
	Sham	MI	Total
1 day	2	2	4
2 day	3	5	8
7 day	1	3	4

Table 3 Number of Differential Genes in Different Infarct Groups

Infarction Time	Number
	Up	Down	Total
1 day	62	0	62
2 day	173	47	220
7 day	602	218	820

Figure 1 Venn diagram of DEGs and protein–protein interaction network. (A) Upregulated genes of DEGs common to all three infarct groups. (B) Downregulated genes of DEGs common to all three infarct groups. (C) Protein–protein interaction network constituted with the DEGs.

Table 4 GO and KEGG Pathway Enrichment Analysis of DEGs

Category	Term	Description	Count	P-value
BP term	GO:0006954	Inflammatory response	10	2.96E-09
BP term	GO:0010628	Positive regulation of gene expression	7	4.21E-05
BP term	GO:0008284	Positive regulation of cell proliferation	7	2.27E-04
BP term	GO:0043066	Negative regulation of apoptotic process	6	0.002199481
BP term	GO:0042127	Regulation of cell proliferation	5	5.13E-04
BP term	GO:0006955	Immune response	5	0.001008589
BP term	GO:0042060	Wound healing	4	4.99E-04
BP term	GO:0006935	Chemotaxis	4	9.67E-04
BP term	GO:0032496	Response to lipopolysaccharide	4	0.00416379
BP term	GO:0071222	Cellular response to lipopolysaccharide	4	0.004910328
BP term	GO:0001525	Angiogenesis	4	0.007114784
BP term	GO:0010629	Negative regulation of gene expression	4	0.009434043
CC term	GO:0005576	Extracellular region	16	2.21E-08
CC term	GO:0005615	Extracellular space	15	2.80E-08
CC term	GO:0070062	Extracellular exosome	13	6.27E-04
CC term	GO:0031012	Extracellular matrix	5	0.001266141
CC term	GO:0009897	External side of plasma membrane	5	0.001669616
MF term	GO:0001968	Fibronectin binding	4	1.32E-05
MF term	GO:0030414	Peptidase inhibitor activity	4	9.85E-04
KEGG pathway	mmu05202	Transcriptional misregulation in cancer	6	3.90E-05
KEGG pathway	mmu05146	Amoebiasis	5	1.65E-04
KEGG pathway	mmu04060	Cytokine–cytokine receptor interaction	5	0.002552568

Table 5 Top 10 Core Genes with Higher Degrees of Connectivity

Gene Symbol	Gene Description	Degree
Il6	Interleukin 6	22
MMP-9	Matrix metallopeptidase 9	20
Spp1	Secreted phosphoprotein 1	12
Ptgs2	Prostaglandin-endoperoxide synthase 2	12
Serpine1	Serine (or cysteine) peptidase inhibitor, clade E, member 1	11
Plaur	Plasminogen activator, urokinase receptor	10
Cxcl5	Chemokine (C-X-C motif) ligand 5	10
Lgals3	Lectin, galactose binding, soluble 3	10
Serpinb2	Serine (or cysteine) peptidase inhibitor, clade B, member 2	9
Cd14	CD14 antigen	9

Figure 2 mRNA and protein expression levels in vivo and in vitro models. (A) The mRNA expression of MMP-9 within the myocardial infarction tissue, (n=3). (B–F) The mRNA expression of MMP-9 within the cardiomyocytes, fibroblasts, endothelial cells, macrophage and neutrophils, (n=3). (G, H) The protein expression of MMP-9 within the myocardial infarction tissue, (n=4). (I, J) The protein expression of MMP-9 within the cardiomyocytes, (n=3). (K, L) The protein expression of MMP-9 within the fibroblasts, (n=3). (M, N) The protein expression of MMP-9 within the endothelial cells, (n=3). (O, P) The protein expression of MMP-9 within the macrophage, (n=3). Data are means ± SDs, *P < 0.05; **P < 0.01.