Bioinformatic analysis of four miRNAs relevant to metastasis-regulated processes in endometrial carcinoma

Figures & data

Table 1 Different expressions of microRNAs between nonmetastasis endometrial cancer and metastasis endometrial cancer

miRNA ID	logFC	AveExpr	t	P-value	Adj. P-value
hsa-mir-1247	1.645411	3.49577	3.696534	0.000263	0.039905
hsa-mir-3200	−1.34271	3.336208	−4.26082	2.78E-05	0.014559
hsa-mir-150	1.218321	8.111698	3.575793	0.000411	0.039905
hsa-mir-301b	−1.2038	1.552897	−3.68286	0.000277	0.039905

Abbreviations: FC, fold change; AveExpr, average expression; Adj. P-value, adjusted P-value.

Table 2 Clinical characteristics of metastasis endometrial cancer patients

Variables	Log numbers	miR-1247 (mean±SD)	P-value	miR-3200 (mean±SD)	P-value	miR-150 (mean±SD)	P-value	miR-301b (mean±SD)	P-value
Age at diagnosis (years)
<60	6	0.75±0.40	0.563	1.05±0.33	0.873	2.13±0.77	0.481	0.67±0.50	0.526
≥60	16	0.90±0.74		1.02±0.45		2.39±0.61		0.52±0.42
Clinical stage
I+II	6	0.93±0.81	0.796	0.81 ±0.20	0.035*	2.37±0.84	0.859	0.43±0.28	0.308
III+IV	16	0.83±0.62		1.11 ±0.44		2.30±0.59		0.61±0.48
T stage
g1+g2	5	0.64±0.90	0.542	0.89±0.32	0.319	2.04±0.98	0.466	0.53±0.62	0.909
g3	17	0.92±0.59		1.07±0.43		2.40±0.53		0.57±0.40
Histologic type
Endometrioid endometrial adenocarcinoma	11	0.85±0.81	0.937	0.98±0.30	0.618	2.24±0.81	0.588	0.62±0.50	0.556
Serous and mixed endometrial adenocarcinoma	11	0.87±0.51		1.07±0.50		2.39±0.47		0.50±0.38
Number of pregnancies
<4	16	0.78±0.64	0.861	1.06±0.40	0.951	2.27±0.68	0.811	0.58±0.50	0.867
≥4	4	0.73±0.46		1.04±0.57		2.33±0.33		0.56±0.13
BMI (kg/m^2)
<28	6	0.84±0.60	0.939	1.14±0.41	0.454	2.41±0.75	0.706	0.65±0.35	0.508
≥28	16	0.86±0.70		0.99±0.41		2.28±0.63		0.53±0.47
Lung metastasis
No	14	1.06±0.65	0.048*	0.93±0.33	0.205	2.38±0.67	0.535	0.55±0.35	0.876
Yes	8	0.51 ±0.54		1.20±0.49		2.20±0.63		0.58±0.58

Note:

* P<0.05.

Abbreviation: BMI, Body Mass Index.

Figure 1 The miR-1247 expression between other organ metastasis tissues and lung metastasis tissues.

Note: *Mean P-value<0.05.

Figure 2 The target gene prediction and function analysis.

Notes: The overlapping target genes were predicted using TargetScan, miRDB, PicTar, and miRanda online analysis tools. (A) miRNA-150; (B) miRNA-301b; (C) miR-1247; and (D) miR-3200.

Figure 3 The GO and KEGG results.

Notes: (A) The significantly enriched KEGG pathways of target genes; (B) the significantly enriched GO biological processes of target genes.

Abbreviations: GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Figure 4 PPI interactions between predicted target genes of miR-301b.

Notes: Yellow color means linkages (in+out) are over than four (hub genes), red means overexpression gene, green means down-expression gene, and blue color means linkages are lower than four.

Abbreviation: PPI, protein–protein interaction.

Figure 5 Expression differences between endometrial cancer and adjacent tissues.

Note: *Mean logFC >1.5, P-value <0.05.

Abbreviations: FC, fold change; UCEC, Uterine Corpus Endometrial Carcinoma.