Figures & data
Figure 1 MTT proliferation assay: DF increases proliferation of LTEP-a-2 and A549 cells.
Notes: (A) Increases proliferation by DF in LTEP-a-2. (B) Increases proliferation by DF in A549. Proliferation of LTEP-a-2 and A549 cells was assessed by MTT proliferation assay, taking the untreated cells as reference. Proliferation was significantly increased in 0.1%, 0.5%, and 1% DF-treated groups compared to the control groups.
Abbreviation: DF, drainage fluid.
Figure 2 Chemosensitivity test: DF weakened the doxycycline and DDP chemosensitivity of lung cancer cells, taking the control groups as reference.
Notes: In the doxycycline chemosensitivity group, viability of LTEP-a-2 cell groups treated with DF (0.1% and 1%) were higher than those of the control groups at both drug concentration levels (5 and 50 mg/L), and the differences were statistically significant (A). Treatment with DDP produced results similar to those obtained with doxycycline (B). DF similarly weakened chemosensitivity of A549 cells (C and D).
Abbreviations: DDP, cisplatin; DF, drainage fluid; N, negative control.
Figure 3 Flow cytometry analysis: LTEP-a-2 and A549 cells in the groups treated with 0.1% and 1% DF showed lower percentages of apoptosis than the control groups.
Notes: (A) Representational figures for apoptosis of LTEP-a-2 and A549 cells. (B) Statistical results for apoptosis of LTEP-a-2 cells. (C) Statistical results for apoptosis of A549 cells.
Abbreviations: DF, drainage fluid; NC, negative control; PI, propidium iodide.
Figure 4 Scratch wound tests: DF promoted migration of LTEP-a-2 and A549 cells.
Notes: Scratch wounds for LTEP-a-2 and A549 cells at 0 and 48 hours after introducing the wound, and treatment with DF. Scratch fold changes in 0.1% and 1% DF-treated groups were significantly higher than in the control group. The scratch closure change area of cells treated with 1% DF tended to be larger than with 0.1% DF.
Abbreviation: DF, drainage fluid.
