The value of the plasma circulating cell-free DNA concentration and integrity index as a clinical tool for prostate cancer diagnosis: a prospective case–control cohort study in an Iranian population

Figures & data

Table 1 Characteristics of the studied groups

	Number of patients	ccfDNA (ALU115) median (range), (ng/μL)	P-value	Integrity (ALU247/ALU115)	P-value
PCa	30	51.03±10.15^a	<0.001	0.35±0.050	<0.001
Age (years)
63.73±8.09	–	–	0.231	–	–
tPSA (ng/mL)
<4	6	68.30±41.36^a	0.024	0.33±0.05	0.737
>4	24	46.71±8.09^a		0.35±0.05
Gleason score
<6	15	52.48±35.51^a	0.723	0.34±0.05	0.724
7	8	38.60±8.25^a		0.36±0.05
8–10	7	62.11±12.11^a		0.36±0.05
Metastatic
Yes	10	98.68±24.81	<0.001	0.36±0.06	0.016
No	20	27.20±3.69		0.34±0.04
BPH	40	13.50±4.40	0.836*	0.16±0.049	0.053
Age (years)
60.80±8.14	–	–	0.231	–	–
tPSA (ng/mL)
<4	28	12.07±3.56	0.083	0.15±0.04	0.091
>4	12	16.83±4.49		0.18±0.05
Healthy subjects	30	9.28±1.51		0.13±0.02
Age (years)
60.63±7.69	–	–	0.231	–	–
tPSA (ng/mL)
<4	30	–	–	–	–
>4	–

Notes: ^aSE; *correlation between BPH group and healthy group.

Abbreviations: BPH, benign prostate hypertrophy; tPSA, total prostate-specific antigen; ccfDNA, circulation cell-free DNA; PCa, prostate cancer.

Figure 1 A number of cancerous samples were examined after amplification by real-time PCR. The products were mixed with loading buffer and loaded on 2% agarose gel. Lanes 1–12 are examined samples. The sequences of ALU247 (above) and ALU115 (at the bottom) are clearly visible. The first and the last wells show the 100 bp DNA marker.

Table 2 Relation between patient’s characteristics with ccfDNA (ALU115) and integrity (ALU247/ALU115) in increased prostate volume and normal prostate volume group

	Number of patients	ccfDNA (ALU115) Median(range) (ng/μL)	P-value	P-value	Integrity (ALU247/ALU115)	P-value	P-value
PCa							0.001
Prostate volume
<35 g (25 cc)	5	49.09	0.956		0.33	0.368
35g (25 cc)–50g (70 cc)	13	50.59			0.34
≫50 g (70 cc)	12	57.12			0.37
BPH
Prostate volume
<35 g (25 cc)	6	12.15	0.161	0.207	0.14	0.363
35 g (25 cc)–50 g (70 cc)	20	13.06			0.16
≫50 g (70 cc)	14	15.60			0.17
Healthy subject
Prostate volume
<35 g (25 cc)	27	–	–		–	–
35 g (25 cc)–50 g (70 cc)	3
≫50 g (70 cc)	0

Abbreviations: PCa, prostate cancer; BPH, benign prostate hypertrophy; ccfDNA, circulation cell-free DNA.