Meta-analysis of the prognostic value of lncRNA DANCR for cancer patients in China

Figures & data

Figure 1 Flowchart presented the steps of study selection in this meta-analysis.

Table 1 The basic information and data of all included studies in the meta-analysis

Reference	Year	Country	Tumor type	Sample size (n)	High lncRNA DANCR expression (n)						Low lncRNA DANCR expression (n)						Reference gene	Cutoff	Detection method
					Total	LTS	PHG	HTS	LNM	DM	Total	LTS	PHG	HTS	LNM	DM
Hao et al^Citation22	2017	China	GC	118	46	6	24	18	20	–	72	37	36	52	48	–	β-actin	–	PCR
Jiang et al^Citation23	2017	China	OSC	34	19	12	–	–	–	12	15	6	–	–	–	5	GAPDH	–	PCR
Li and Zhou^Citation24	2018	China	Glioma	86	43	–	–	38	–	–	43	–	–	21	–	–	GAPDH	Mean	PCR
Liang et al^Citation25	2019	China	CVR	65	33	23	–	21	18	–	32	11	–	7	4	–	GAPDH	Median	PCR
Liu et al^Citation26	2015	China	CRC	104	52	24	29	40	21	–	52	21	14	27	8	–	GAPDH	Median	PCR
Mao et al^Citation27	2017	China	GC	60	30	13	–	18	23	–	30	16	–	9	13	–	GAPDH	Median	PCR
Pan et al^Citation28	2018	China	GC	65	40	27	25	33	36	1	25	6	17	13	12	0	U6	–	PCR
Sha et al^Citation29	2017	China	TNBC	63	32	10	19	20	17	–	31	10	6	6	5	–	GAPDH	Median	PCR
Wang et al^Citation30	2018	China	RB	57	29	–	–	–	–	–	28	–	–	–	–	–	GAPDH	Median	PCR
Wang et al^Citation31	2018	China	CRC	47	26	20	8	21	22	23	21	11	6	8	11	9	GAPDH	–	PCR
Wang et al^Citation32	2018	China	OSC	95	72	49	–	46	–	50	23	7	–	7	–	8	GAPDH	Mean	PCR
Yang et al^Citation33	2018	China	Glioma	82	41	–	–	–	–	–	41	–	–	–	–	–	GAPDH	Median	PCR
Yuan et al^Citation34	2016	China	HCC	135	68	–	–	–	–	–	67	–	–	–	–	–	GAPDH	Median	PCR
Zhan et al^Citation35	2018	China	BC	106	70	44	45	59	11	–	36	20	13	21	3	–	β-actin	–	PCR

Note: The dashes represent no data.

Abbreviations: BC, bladder cancer; CRC, colorectal cancer; CVR, cervical cancer; DM, distant metastasis; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; HCC, hepatocellular carcinoma; HTS, high tumor stage; lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA; LNM, lymph node metastasis; LTS, larger tumor size; OSC, osteosarcoma; PHG, poor histological grade; RB, retinoblastoma; TNBC triple negative breast cancer.

Figure 2 Forest plot (A) and sensitivity analysis (B) showed the relationship between lncRNA DANCR expression level and OS in cancer.

Abbreviations: lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA; OS, overall survival.

Figure 3 Forest plots of subgroup analysis for OS of patients with cancer.

Notes: Subgroup analysis by tumor type (A), sample size (B), NOS score (C), and HR statistic method (D).

Abbreviations: NOS, Newcastle–Ottawa Scale; OS, overall survival.

Table 2 Survival data of studies included in the meta-analysis

Reference	Year	Country	Tumor type	Sample size (n)	Method	OS, HR (95% CI)	DFS, HR (95% CI)	HR statistic	NOS
Hao et al^Citation22	2017	China	GC	118	Multivariate	1.66 (1.0363–2.6590)	NA	Survival curve	8
Jiang et al^Citation23	2017	China	OSC	34	Multivariate	5.65 (1.565–20.408)	3.759 (1.179–12.048)	Data in paper	7
Li and Zhou^Citation24	2018	China	Glioma	86	Multivariate	1.85 (1.0844–3.1562)	NA	Survival curve	7
Liang et al^Citation25	2019	China	CVR	65	Multivariate	2.06 (1.0683–3.9724)	NA	Survival curve	7
Liu et al^Citation26	2015	China	CRC	104	Multivariate	2.131 (1.157–7.058)	2.397 (1.385–7.279)	Data in paper	8
Mao et al^Citation27	2017	China	GC	60	NA	NA	NA	NA	8
Pan et al^Citation28	2018	China	GC	65	NA	NA	NA	NA	7
Sha et al^Citation29	2017	China	TNBC	63	Multivariate	1.56 (1.02–2.38)	NA	Survival curve	8
Wang et al^Citation30	2018	China	RB	57	Multivariate	2.26 (1.2694–4.0238)	2.84 (1.3068–6.1721)	Survival curve	6
Wang et al^Citation31	2018	China	CRC	47	NA	NA	NA	NA	8
Wang et al^Citation32	2018	China	OSC	95	Multivariate	1.66 (1.2037–2.2893)	NA	Survival curve	7
Yang et al^Citation33	2018	China	Glioma	82	Multivariate	1.783 (1.121–3.4821)	NA	Data in paper	6
Yuan et al^Citation34	2016	China	HCC	135	Multivariate	2.757 (1.379–5.514)	2.228 (1.359–3.653)	Data in paper	6
Zhan et al^Citation35	2018	China	BC	106	NA	NA	NA	NA	7

Note: NA represents no data.

Abbreviations: BC, bladder cancer; CRC, colorectal cancer; CVR, cervical cancer; DFS, disease-free survival; GC, gastric cancer; HCC, hepatocellular carcinoma; NOS, Newcastle–Ottawa Scale; OS, overall survival; OSC, osteosarcoma; RB, retinoblastoma; TNBC triple negative breast cancer.

Table 3 Subgroup analysis of OS by tumor type, sample size, NOS score, and HR statistic method

Subgroup analysis	No. of studies	No. of patients	Pooled HR (95% CI)	Heterogeneity
				I^2 (%)	P-value
Total	10	839	1.85 (1.56–2.18)	0.0	0.728
Cancer type
Digestive system cancer	3	357	1.98 (1.38–2.83)	0.0	0.487
Non-digestive system cancer	7	482	1.81 (1.50–2.19)	0.0	0.610
Sample size
Number >90	4	452	1.79 (1.41–2.28)	0.0	0.584
Number ≤90	6	387	1.90 (1.50–2.40)	0.0	0.540
NOS score
NOS >7	3	285	1.65 (1.23–2.23)	0.0	0.829
NOS ≤7	7	554	1.94 (1.59–2.38)	0.0	0.548
HR statistic
Survival curve	6	484	1.75 (1.45–2.11)	0.0	0.917
Data in paper	4	355	2.31 (1.59–3.37)	0.0	0.400

Abbreviations: NOS, Newcastle–Ottawa Scale; OS, overall survival.

Figure 4 Forest plot showed the relationship between lncRNA DANCR expression level and DFS in cancer.

Abbreviations: DFS, disease-free survival; lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA.

Figure 5 Forest plot (A), sensitivity analysis (B), and the forest plot of sensitivity analysis (C) showed the association between LTS and lncRNA DANCR expression level in cancer.

Abbreviations: lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA; LTS, larger tumor size.

Figure 6 Forest plot (A), sensitivity analysis (B), and the forest plot of sensitivity analysis (C) showed the association between PHG and lncRNA DANCR expression level in cancer.

Abbreviations: lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA; PHG, poor histological grade.

Figure 7 Forest plot (A), sensitivity analysis (B), and the forest plot of sensitivity analysis (C) showed the association between HTS and lncRNA DANCR expression level in cancer.

Abbreviations: HTS, high tumor stage; lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA.

Figure 8 Forest plot (A), sensitivity analysis (B), and the forest plot of sensitivity analysis (C) showed the association between LNM and lncRNA DANCR expression level in cancer.

Abbreviations: lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA; LNM, lymph node metastasis.

Figure 9 Evaluation of the relationship between lncRNA DANCR expression level and DM in cancer.

Abbreviations: DM, distant metastasis; lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA.

Figure 10 Begg’s publication bias plots evaluating the relationship between lncRNA DANCR expression and OS (A), DFS (B), LTS (C), PHG (D), HTS (E), LNM (F), DM (G).

Abbreviations: DFS, disease-free survival; DM, distant metastasis; HTS, high tumor stage; lncRNA DANCR, long non-coding RNA anti-differentiation noncoding RNA; LNM, lymph node metastasis; LTS, larger tumor size; OS, overall survival; PHG, poor histological grade.

HaoYPQiuJHZhangDBYuCGLong non-coding RNA DANCR, a prognostic indicator, promotes cell growth and tumorigenicity in gastric cancerTumour Biol2017396101042831769979828618943

 PubMedGoogle Scholar

JiangNWangXXieXlncRNA DANCR promotes tumor progression and cancer stemness features in osteosarcoma by upregulating Axl via miR-33a-5p inhibitionCancer Lett2017405465528642170

 PubMed Web of Science ®Google Scholar

LiJZhouLOverexpression of lncRNA DANCR positively affects progression of glioma via activating Wnt/β-catenin signalingBiomed Pharmacother201810260260729602127

 PubMed Web of Science ®Google Scholar

LiangHZhangCGuanHLiuJCuiYLncRNA DANCR promotes cervical cancer progression by upregulating ROCK1 via sponging miR-335-5pJ Cell Physiol201923457266727830362591

 PubMed Web of Science ®Google Scholar

LiuYZhangMLiangLLiJChenYXOver-expression of lncRNA DANCR is associated with advanced tumor progression and poor prognosis in patients with colorectal cancerInt J Clin Exp Pathol201589114801148426617879

 PubMed Web of Science ®Google Scholar

MaoZLiHDuBLncRNA DANCR promotes migration and invasion through suppression of lncRNA-LET in gastric cancer cellsBiosci Rep2017376BSR2017107028951520

 PubMed Web of Science ®Google Scholar

PanLLiangWGuJLong noncoding RNA DANCR is activated by SALL4 and promotes the proliferation and invasion of gastric cancer cellsOncotarget2018921915193029416741

 PubMedGoogle Scholar

ShaSYuanDLiuYHanBZhongNTargeting long non-coding RNA DANCR inhibits triple negative breast cancer progressionBiol Open2017691310131628760736

 PubMed Web of Science ®Google Scholar

WangJXYangYLiKLong noncoding RNA DANCR aggravates retinoblastoma through miR-34c and miR-613 by targeting MMP-9J Cell Physiol2018233106986699529744877

 PubMed Web of Science ®Google Scholar

WangYLuZWangNLong noncoding RNA DANCR promotes colorectal cancer proliferation and metastasis via miR-577 spongingExp Mol Med20185055729717105

 PubMedGoogle Scholar

WangYZengXWangNLong noncoding RNA DANCR, working as a competitive endogenous RNA, promotes ROCK1-mediated proliferation and metastasis via decoying of miR-335-5p and miR-1972 in osteosarcomaMol Cancer20181718929753317

 PubMedGoogle Scholar

YangJXSunYGaoLMengQYangBYLong non-coding RNA DANCR facilitates glioma malignancy by sponging miR-33a-5pNeoplasma201865579079829940760

 PubMed Web of Science ®Google Scholar

YuanSXWangJYangFLong noncoding RNA DANCR increases stemness features of hepatocellular carcinoma by derepression of CTNNB1Hepatology201663249951125964079

 PubMed Web of Science ®Google Scholar

ZhanYChenZLiYLong non-coding RNA DANCR promotes malignant phenotypes of bladder cancer cells by modulating the miR-149/MSI2 axis as a ceRNAJ Exp Clin Cancer Res201837127330419948

 PubMedGoogle Scholar