https://orcid.org/0000-0002-1715-0887
Figures & data
Figure 1 Up-regulation of FOXD2-AS1 in CRC predicts poor survival. qPCR was performed to measure the expression levels of FOXD2-AS1 in both CRC and non-tumor tissues derived from the 60 CRC patients included in this study (A). Survival curves were performed following steps: 1) dividing the patients into high and low FOXD2-AS1 level groups (n=30) with the median expression level in CRC as cutoff value; 2) survival curve plotting by Kaplan-Meier plotter; 3) survival curve comparison by log rank test (B). PCR reactions were repeated 3 times and mean values were presented. ***p<0.0001.
Figure 2 MiR-25-3p may bind FOXD2-AS1 but failed to affect its expression. IntaRNA (http://rna.informatik.uni-freiburg.de/IntaRNA/Input.jsp) was used to predict the interaction between miR-25-3p and FOXD2-AS1. It was observed that miR-25-3p can bind FOXD2-AS1 and form strong base pairing (A). To further analyze the relationship between them, CR4 cells were transfected with FOXD2-AS1 vector or miR-25-3p mimic. Over-expression of FOXD2-AS1 and miR-25-3p was confirmed at 24h post-transfection by qPCR (B). Moreover, the effects of FOXD2-AS1 over-expression on miR-25-3p (C) and the effects of miR-25-3p over-expression on FOXD2-AS1 (D) were analyzed by qPCR. Experiments were repeated 3 times and data were expressed as mean values. *p<0.05.
Figure 3 FOXD2-AS1 over-expression up-regulated miR-25-3p target gene Sema4C. Sema4C is a target gene of miR-25-3p. Therefore, qPCR and Western blot experiments were performed to analyze the effects of miR-25-3p and FOXD2-AS1 over-expression on the expression of Sema4C at both mRNA (A) and protein (B) levels. Experiments were repeated 3 times and data were expressed as mean values. *p<0.05.
Figure 4 FOXD2-AS1 regulated miR-25-3p/Sema4C axis to promote CR4 cell invasion and migration. Transwell invasion and migration assays were performed to analyze the effects of FOXD2-AS1, miR-25-3p and Sema4C over-expression on the invasion (A) and migration (B) of CR4 cells. Experiments were repeated 3 times and data were expressed as mean values. *p<0.05.
