Figures & data
Figure 1 LINC01121 expression levels were significantly in breast cancer cell lines compared with MCF-10A. (A) The structure of LINC01121 (1095bp), such as chromosomal locations and exons, was diagrammed. (B) LINC01121 expression levels in breast cancer cell lines (MCF-7, BT-549, MDA-MB-231, and MDA-MB-453) and the normal human breast cell line (MCF-10A) were measured by qRT-PCR after cultured at 24 h. ***p < 0.001, vs MCF-10A cells. (C) LINC01121 expression was significantly higher in cytoplasm than that in nuclear in MCF-7 cell. (D) LINC01121 expression was significantly higher in cytoplasm than that in nuclear in MDA-MB-231 cell.
Figure 2 LINC01121 down-regulation significantly suppressed proliferation in MCF-7 and MDA-MB-231 cells (A) LINC01121 expression levels in MCF-7 and MDA-MB-231 cells after transfected at 48 h si-LINC01121-1/2/3 was measured by qRT-PCR. (B and C) Proliferation of MCF-7 and MDA-MB-231 cells was determined by MTS assay after transfected si-LINC01121 at 48 h (si-LINC01121-1) (***p < 0.001).
Figure 3 LINC01121 down-regulation significantly suppressed cell cycle progression and promoted apoptosis in MCF-7 and MDA-MB-231 cells. (A and B) Cell cycle progression and apoptosis in MCF-7 and MDA-MB-231 cells were assessed using flow cytometry after transfected si-LINC01121 at 48 h (*P<0.05, ***p < 0.001).
Figure 4 LINC01121 down-regulation suppressed MCF-7 and MDA-MB-231 cell migration and invasion. (A and B) Migration and invasion of MCF-7 and MDA-MB-231 cells was determined by transwell experiments after transfected si-LINC01121 at 48 h (***p < 0.001).
Figure 5 LINC01121 directly bound to miR-150-5p and indirectly affected HMGA2 expression (A) The predicted binding site between LINC01121 and miR-150-5p. (B) Luciferase reporter assays demonstrated that LINC01121 directly bound to miR-150-5p. (C) The relative expression levels of miR-150-5p in breast cancer cells (MCF-7, BT-549, MDA-MB-231, and MDA-MB-453) and the normal breast epithelial cell line (MCF-10A) were measured by qRT-PCR. (D) The expression of HMGA2 protein in MCF-7, BT-549, MDA-MB-231, MDA-MB-453 and MCF-10A cells was measured by Western blotting. (E) The expression of miR-150-5p in MCF-7 and MDA-MB-231 cells was measured by qRT-PCR after transfected si-LINC01121 at 48 h. (F) The expression of HMGA2 in MCF-7 and MDA-MB-231 cells was measured by Western blotting after transfected si-LINC01121 at 48 h (***p < 0.001).
Figure 6 miR-150-5p knockdown significantly attenuated the effects of LINC01121 silencing on HMGA2 protein expression and cell proliferation in breast cancer cells (A) The relative expression levels of miR-150-5p in MCF-7 and MDA-MB-231 cells were measured by qRT-PCR after co-transfected miR-150-5p inhibitor and si-LINC01121 or co-transfected with an NC inhibitor and si-LINC01121 at 48 h. (B) HMGA2 protein expression in MCF-7 and MDA-MB-231 cells was measured by Western blotting after co-transfected miR-150-5p inhibitor and si-LINC01121 or co-transfected with an NC inhibitor and si-LINC01121 at 48 h. (C and D) Proliferation of MCF-7 and MDA-MB-231 cells was measured by MTS assay after co-transfected miR-150-5p inhibitor and si-LINC01121 or co-transfected with an NC inhibitor and si-LINC01121 at 48 h (**p < 0.01, ***p < 0.001).
Figure 7 miR-150-5p knockdown significantly attenuated the effects of LINC01121 silencing on cell cycle progression and apoptosis in breast cancer cells. (A and B) The effect of miR-150-5p knockdown on cell cycle progression and apoptosis in MCF-7 and MDA-MB-231 cells was assessed by flow cytometry after co-transfected miR-150-5p inhibitor and si-LINC01121 or co-transfected with an NC inhibitor and si-LINC01121 at 48 h (*P<0.05, ***p < 0.001).
Figure 8 miR-150-5p knockdown significantly attenuated the repressive effects of LINC01121 down-regulation on the migration and invasion of breast cancer cells (A and B) Migration and invasion of MCF-7 and MDA-MB-231 cells were measured by transwell after co-transfected miR-150-5p inhibitor and si-LINC01121 or co-transfected with an NC inhibitor and si-LINC01121 at 48 h (***p < 0.001).
