Figures & data
Figure 1 MYOSLID was upregulated in OS. (A) MYOSLID expression in OS tissues and adjacent normal tissues by qRT-PCR. (B) Relative expression of MYOSLID in OS cell lines. (C) The Kaplan–Meier curve was used to determine overall survival rate according to MYOSLID expression. **P<0.01 and ***P<0.001.
Figure 2 Effects of MYOSLID on proliferation, migration and invasion. (A) Relative expression of MYOSLID in Saos2 and U2OS cells. (B, C) CCK8 assay and colony formation assay were performed to measure proliferation. (D, E) MYOSLID knockdown inhibited migration and invasion by Transwell assay. **P<0.01 and ***P<0.001.
Figure 3 MYOSLID sponged miR-1286. (A) Interaction prediction between MYOSLID and miR-1286 by miRDB. (B) miR-1286 was overexpressed by transfection with miR-1286 mimics. (C) Luciferase reporter assay in U2OS cells by transfection with miR-1286 mimics and MYOSLID reporters. (D) RNA pulldown assay showed that biotin labeled miR-1286-WT interacted with MYOSLID. (E) Relative expression of miR-1286 after MYOSLID knockdown. (F) miR-1286 was downregulated in OS tissues. (G) Expression correlation between MYOSLID and miR-1286 in OS tissues. ***P<0.001.
Figure 4 miR-1286 directly targeted RAB13. (A) Interaction prediction between miR-1286 and RAB13 by TargetScan. (B) Luciferase reporter assay in U2OS cells by transfection with miR-1286 mimics and RAB13 reporters. (C, D) RIP assay showed that miR-1286 and RAB13 were co-precipitated by anti-Ago2. (E) Relative expression of RAB13 after miR-1286 mimic transfection. (F) Relative expression of RAB13 after si-MYOSLID and miR-1286 mimic transfection. ***P<0.001.
Figure 5 MYOSLID promoted OS progression via miR-1286/RAB13 axis. (A) Relative expression of RAB13 after transfection of indicated plasmids. (B) Cell proliferation was measured by CCK8 assays. (C, D) Migration and invasion were determined by Transwell assays. **P<0.01 and ***P<0.001.
