https://orcid.org/0000-0003-2230-5465
Figures & data
Figure 1 Knockdown of TGIF1 inhibited growth capacity of gastric cancer cells.
Notes: (A and B) Four TGIF1 siRNAs (si-RNA1, si-RNA2, si-RNA3 and si-RNA4) were transfected into gastric cancer cells respectively. TGIF1 mRNA expression level was detected with qPCR. (C) TGIF1 si-RNA3 was transfected into AGS and MKN45 cells to generate TGIF1 knockdown cell model (si-TGIF1). TGIF1 protein expression level was evaluated by Western blotting. (D and E) CCK8 assay was performed to assess the cell proliferation. (F) Plate clone formation test. *P<0.05, **P<0.01. Results are from three repeated experiments.
Figure 2 TGIF1 knockdown inhibited the invasion and migration of gastric cancer cells.
Notes: (A and B) Transwell assay was used to evaluated the invasion and migration of AGS and MKN45 cells. (C) Wound-healing assay was performed to detect the migration of cells. (D) Gelatinase experiment for MMP-2. *P<0.05, **P<0.01. Results are from three repeated experiments.
Figure 3 TGIF1 knockdown promoted the apoptosis of gastric cancer cells.
Notes: (A) Cell apoptosis was evaluated using flow cytometry assay. (B) Hoechst-PI double staining of apoptosis. (C) The expression of apoptosis-related proteins (Bcl-2, Bax, and active caspase3) was analyzed with Western blotting. *P<0.05, **P<0.01. Results are from three repeated experiments.
Figure 4 TGIF1 affected the AKT signaling pathway in gastric cancer cells.
Notes: (A) Cell cycle was evaluated using flow cytometry assay. (B) Western blot assay was used to analyze the effect of TGIF1 on AKT signaling pathway. *P<0.05, **P<0.01. Results are from three repeated experiments.
Table 1 Clinical Significance of TGIF1 Was Obtained Using LinkedOmics Based Analysis