Figures & data
Table 1 Correlations of LINC00665 Expression and Clinicopathological Features of Breast Cancer Patients
Table 2 Univariate and Multivariate Analyses of Overall Survival in Patients with BC
Figure 1 LINC00665 was upregulated in BC samples and cell lines. (A) LINC00665 was upregulated in BC samples collected compared with normal samples. (B) LINC00665 was upregulated in BC tissues compared with normal sample detected at GEPIA. (C) LINC00665 was upregulated in BC tissues compared with normal sample detected at ENCORI. (D) High LINC00665 was a predictor for poorer overall survival of BC patients. * P < 0.05 compared with normal tissues.
Figure 2 Knocking down LINC00665 suppresses BC cell proliferation, but induces apoptosis. (A) LINC00665 was upregulated in BC cells compared with normal cells. (B) After si-LINC00665 transfection, LINC00665 expression in BC cells was down-regulated. (C) After LINC00665 knockdown, the proliferation of BC cells was inhibited. (D) After LINC00665 knockdown, the colony formation ability of BC cells was inhibited. (E) After LINC00665 knockdown, the apoptosis of BC cells was enhanced. ***P < 0.001, **P < 0.01 compared with si-NC group.
Figure 3 LINC00665 interacts with miR-551b-5p. (A) Potential targeting sites of LINC00665 and miR-551b-5p. (B) Transfection of miR-551b-5p mimic increased miR-551b-5p levels in BC cells. mi-NC group was used as control. (C) Transfection of miR-551b-5p inhibitor decreased miR-551b-5p levels in BC cells. mi-NC group was used as control. (D) Relative luciferase activity assay showed miR-551b-5p overexpression decreased relative luciferase activity in BC cells with LINC00665-WT transfection. mi-NC group was used as control. (E) RNA pull-down assay indicates the interactions of LINC00665 and miR-551b-5p. BIO-mi-NC group was used as control. (F) Transfection of si-LINC00665 increased miR-551b-5p levels in BC cells. si-NC group was used as control. (G) Negatively correlation of LINC00665 and miR-551b-5p in BC tissues. ***P < 0.001.
Figure 4 miR-551b-5p was down-regulated in BC samples and cell lines. (A) miR-551b-5p was downregulated in BC samples collected compared with normal samples. Normal tissues were used as control. (B) miR-551b-5p was downregulated in BC cells compared with normal cells. MCF10A cells were used as control. (C) miR-551b-5p was downregulated in BC tissues compared with normal sample detected at UALCAN. Normal tissues were used as control. (D) Low miR-551b-5p was a predictor for poorer overall survival of BC patients. ***P < 0.001, **P < 0.01.
Figure 5 LINC00665 regulates BC cell proliferation and apoptosis via miR-551b-5p. (A) miR-551b-5p expression after transfection of si-LIN00665 and miR-551b-5p inhibitor. (B) Proliferation of BC cells after transfection of si-LIN00665 and miR-551b-5p inhibitor. (C) Colony formation ability of BC cells transfected with si-LIN00665 and miR-551b-5p inhibitor. (D) Apoptosis of BC cells transfected with si-LIN00665 and miR-551b-5p inhibitor. ***P < 0.001, **P < 0.01, *P < 0.05 compared with si-NC+mi-NC group.
Figure 6 Effect of LINC00665 on BC in vivo. (A) Tumor volume of different experimental groups at different time points. (B) Tumor weight of different experimental groups. (C) Relative gene expression in different experimental groups. ***P < 0.001 compared with sh-NC group.
